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Indoor sprouting experiment method for preventing seeds from molding

A germination test and seed technology, which is applied in the field of agriculture, can solve the problems of inability to germinate seeds, lack of oxygen, and easy moldy seeds, etc., and achieve the effects of simple flow-through operation, prevention of seed moldy, and wide application range

Inactive Publication Date: 2015-11-25
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(2) The temperature is not suitable for the germination test
(3) Inadvertent ventilation during the test period caused the seeds to lack oxygen, which was not conducive to seed germination
(4) Untimely treatment of moldy and rotten seeds during seed germination will cause fungal infection and affect test results
The main reason is that the temperature in the incubator is high, the humidity is high, it is easy to be infected with mold, the air is not fresh, and some seeds cannot germinate due to mildew.
[0003] The following difficult problems often appear in the indoor seed germination test: (1) The seeds are prone to mold and infection
(2) Poor ventilation conditions make it difficult for plants to breathe, resulting in failure to germinate or rot
(3) Lack of water in the middle and late stages of germination, and the germination box is dry and sometimes wet, which is not conducive to the healthy growth and development of seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A kind of indoor germination test method that prevents seed from going moldy, comprises the following steps:

[0027] 1) Seed disinfection: Put the seeds into a seed mesh bag and rinse with water for 1 hour, soak in 500 times chlorothalonil solution for 12 hours, then sterilize with 10% sodium hypochlorite for 10 minutes, and wash with sterile water for 5 times;

[0028] 2) Plastic gloves for disinfection: soak in 500 times of chlorothalonil for 12 hours, then disinfect with 10% sodium hypochlorite for 10 minutes, and wash with sterile water for 5 times;

[0029] 3) High temperature and high pressure sterilization of river sand: Rinse the sieved river sand without harmful substances with clean water for 1 hour, wrap it in two layers with newspaper, put it in an autoclave, sterilize it at 150°C for 30 minutes, and then cool it down to room temperature naturally ;The particle size of river sand is 0.05~0.08 mm;

[0030] 4) Dormant seeds need low-temperature stratificatio...

Embodiment 2

[0035] A kind of indoor germination test method that prevents seed from going moldy, comprises the following steps:

[0036] 1) Seed disinfection: Put the seeds into a seed mesh bag and rinse with water for 1 hour, soak in 500 times potassium permanganate solution for 12 hours, then sterilize with 10% sodium hypochlorite for 10 minutes, and wash with sterile water for 5 times;

[0037] 2) Plastic gloves for disinfection: soak in 500 times potassium permanganate solution for 12 hours, then disinfect with 10% sodium hypochlorite for 10 minutes, and wash with sterile water for 5 times;

[0038] 3) High temperature and high pressure sterilization of river sand: Rinse the sieved river sand without harmful substances with clean water for 1 hour, wrap it in two layers with newspaper, put it in an autoclave, sterilize it at 150°C for 30 minutes, and then cool it down to room temperature naturally ;The particle size of river sand is 0.05~0.08 mm;

[0039] 4) Dormant seeds need low-tem...

Embodiment 3

[0044] A kind of indoor germination test method that prevents seed from going moldy, comprises the following steps:

[0045] 1) Seed disinfection: Put the seeds into a seed mesh bag and rinse with water for 1 hour, soak in 500 times formaldehyde solution for 12 hours, then sterilize with 10% sodium hypochlorite for 10 minutes, and then wash with sterile water 5 times;

[0046] 2) Plastic gloves for disinfection: soak in 500 times formaldehyde for 12 hours, then disinfect with 10% sodium hypochlorite for 10 minutes, and then wash with sterile water for 5 times;

[0047] 3) High temperature and high pressure sterilization of river sand: Rinse the sieved river sand without harmful substances with clean water for 1 hour, wrap it in two layers with newspaper, put it in an autoclave, sterilize it at 150°C for 30 minutes, and then cool it down to room temperature naturally ;The particle size of river sand is 0.05~0.08 mm;

[0048] 4) Dormant seeds need low-temperature stratification...

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PUM

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Abstract

The invention discloses an indoor sprouting experiment method for preventing seeds from molding. The method comprises steps as follows: (1), disinfecting seeds; (2), disinfecting plastic gloves for standby use; (3), performing high-temperature and high-pressure sterilization on river sand; (4), performing cold stratification processing on dormant seeds; (5), sowing; (6), putting the seeds into a disinfected artificial intelligent climate chamber for sprouting. In order to overcome the defect of serious molding of seeds in a laboratory in the sprouting process currently, the indoor sprouting experiment method is adopted, seed disinfection, high-temperature and high-pressure sterilization of the river sand and uniform mixing of the seeds and the wet river sand are performed, so that the seeds keep moisture uniformly, external conditions required in a seed sprouting test are adjusted through the artificial intelligent climate chamber, and a knob for controlling the size of an air hole is arranged on a cover of a preservation box to adjust moisture, temperature and oxygen required by the seeds in the sprouting process. The relation among moisture, temperature and oxygen required by the seeds can be adjusted, the seeds can be effectively prevented from molding, the sprout rate of the seeds is increased, and the reliability and the accuracy of test data are improved. Multiple tests prove that the effect is remarkable, and molding phenomena seldom occur.

Description

technical field [0001] The invention relates to the technical field of agriculture, in particular to an indoor germination test method for preventing seeds from becoming moldy. Background technique [0002] One of the common problems in the germination test is that the control of the germination test conditions is not in place. (1) Too much or too little water was added during the germination test. Too much water will cause insufficient oxygen, and the seeds will rot; too little water will also affect imbibition and germination. (2) The temperature is not suitable for the germination test. Appropriate temperature can promote seed germination. If the temperature is too high, various enzyme systems will be damaged to varying degrees, causing internal metabolic disorders and making it difficult for seeds to germinate. Seeds germinate and are therefore susceptible to disease damage. (3) During the test period, lack of attention to ventilation caused the seeds to lack oxygen,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02
Inventor 刘春风周余华王磊张虎王红梅
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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