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Method for preventing deterioration of unsensitized latex reagent

A reagent and degradation technology, applied in biological testing, material inspection products, measuring devices, etc., can solve the problems such as betaine addition that are not yet known

Inactive Publication Date: 2015-12-02
FUJIREBIO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, for latex reagents, there is no known case where betaine is added to the latex for protection from freezing deterioration.

Method used

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  • Method for preventing deterioration of unsensitized latex reagent
  • Method for preventing deterioration of unsensitized latex reagent
  • Method for preventing deterioration of unsensitized latex reagent

Examples

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Embodiment

[0058] Hereinafter, the present invention will be described more specifically based on examples. However, the present invention is not limited by the following examples.

[0059]

[0060] About 5 mL of an unsensitized latex reagent (hereinafter R1 reagent) and an anti-HbA1c antibody solution (hereinafter R2 reagent) contained in a known HbA1c immunoassay kit were dispensed into 10 mL tubes. The tubes were stored overnight in a freezer at -20°C so that the R1 and R2 reagents were frozen. These were left to stand at room temperature, thawed, and the status of the reagents was checked visually.

[0061] For the R1 reagent, the latex produces a white coagulated precipitate, and the precipitated liquid becomes transparent. As an immunoassay reagent, it is obviously in an unusable state. On the other hand, the R2 reagent showed no change in appearance. Therefore, the R2 reagent frozen?thawed 0 times, the R2 reagent frozen?thawed 1 time and the R1 reagent frozen?thawed 0 times ...

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Abstract

Provided is a novel means for suitably maintaining the dispersion state of a latex reagent in an immunoassay kit and preventing the occurrence of coagulation and sedimentation due to changes in temperature so that a reagent is protected from deterioration in measurement performance, said means also being applicable to immunoagglutination measurement kits in which a small amount of a latex reagent is dispensed. An immunoassay reagent according to the present invention comprises unsensitized latex particles and trimethyl glycine in a solvent. The concentration of trimethyl glycine in the reagent is preferably 5-10 w / v%. The reagent can be suitably used as an immunoassay reagent for hemoglobin A1c, for example.

Description

technical field [0001] The present invention relates to a method for preventing performance deterioration of an immunoassay reagent using non-sensitized latex. Background technique [0002] In the so-called latex reagent in an immunoassay kit, an antibody that specifically reacts with a substance to be measured or an antigen substance that reacts with an antibody to be measured is usually immobilized in advance and supported on a latex surface. In order to improve the storage stability of such latex reagents, lyophilization has been attempted by adding sugars and / or surfactants (Patent Documents 1 and 2). According to this method, stable storage of the latex reagent can be realized. However, when such a freeze-drying method is used, it is necessary to reconstitute the latex emulsion before use, which is not convenient for users. [0003] In consideration of user convenience, a liquid latex reagent that can be used as it is is ideal. As a liquid latex reagent that does not...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/545G01N33/72
CPCG01N33/72G01N33/531G01N33/5306G01N33/723
Inventor 铁本融平田稔
Owner FUJIREBIO CO LTD
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