Method for fixing eyeball of living mouse

A technology of mice and eyeballs, which is applied in the fields of animal restraint equipment, medical science, veterinary equipment, etc., can solve the problems of inability to perform follow-up operations, etc., and achieve the goal of overcoming complex processes and extremely low success rate, simple operation, and low cost Effect

Inactive Publication Date: 2015-12-30
WUXI NO 2 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the mouse eyeball is small and the eyeball can rotate 360 ​​degrees, it is impossible to perform precise follow-up operations when the eyeball is not fixed

Method used

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Examples

Experimental program
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Embodiment Construction

[0018] A method for fixing eyeballs of living mice is characterized by the following steps:

[0019] 1. Anesthetize the mice with 4.3% chloral hydrate at 0.1ml / 10g. After 5 minutes, observe that the mice's muscles are relaxed, breathing is stable, and corneal reflex is slow.

[0020] 2. Fix the mouse limbs on the flat board with clamps, and wipe around the mouse eyelids with iodophor.

[0021] A. Visually observe the diameter of the mouse eyeball to be fixed, and then use tweezers or needle-nose pliers to adjust the diameter of the ring structure to be slightly larger or consistent with the diameter of the mouse eyeball.

[0022] 3. Under the electron microscope, pinch the handle of the mouse eye snare with the index finger and thumb of the left hand, press the ring structure of the mouse eye snare from top to bottom, and press the ring structure directly against the upper surface of the mouse eyelid. On the upper surface of the mouse eyelid, the ring structure first touches the mouse...

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Abstract

A method for fixing the eyeball of a living mouse comprises the steps that 1, the mouse is anesthetized with 4.3% chloral hydrate based on the rate of 0.1ml/10g, and it can be observed that all muscles of the mouse relax, breathing is steady, and corneal reflex is blunt five minutes after anesthetization; 2, the limbs of the mouse are fixed to a surface plate with clips, and the area around the eyelid of the mouse is scrubbed with iodophor; 3, under an electron microscope, a handle of a mouse eyeball snare is held with the index finger and thumb of the left hand, the annular structure of the mouse eyeball snare is pressed down from top to bottom right facing the upper surface of the eyelid of the mouse, the eyeball of the mouse is trapped and fixed by the annular structure after the eyelid of the mouse is pushed away by the annular structure, the eyeball of the mouse protrudes upwards out of the plane of the annular structure at the moment, and the annular structure is pressed downwards continuously till veins on the sclera of the eyeball of the mouse are exposed clearly.

Description

Technical field [0001] The invention relates to a method for fixing eyeballs of living mice. Background technique [0002] For various reasons, it is necessary to perform operations and treatments on the eyeballs of living mice, such as measuring intraocular pressure in mice, observation, measurement and experiments on the structure of various organs and tissues on the surface of the mouse eyeball, including the sclera and sclera of the mouse eyeball Perform, measure, experiment or follow-up other treatments on upper surface veins. Since mice have small eyeballs and their eyeballs can rotate 360 ​​degrees, precise follow-up operations cannot be performed when the eyeballs are not fixed. Summary of the invention [0003] The invention aims to provide a method for fixing eyeballs of living mice. [0004] First introduce the tool used, that is, a mouse eye snare described in Chinese Patent ZL201520730313.2, which includes a vertical handle made of stainless steel and a ring structure...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D3/00
Inventor 罗莎莎武志峰施超邹文军
Owner WUXI NO 2 PEOPLES HOSPITAL
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