A method for fixing the eyeball of a living mouse comprises the steps that 1, the mouse is anesthetized with 4.3% chloral hydrate based on the rate of 0.1ml/10g, and it can be observed that all muscles of the mouse relax, breathing is steady, and corneal reflex is blunt five minutes after anesthetization; 2, the limbs of the mouse are fixed to a surface plate with clips, and the area around the eyelid of the mouse is scrubbed with iodophor; 3, under an electron microscope, a handle of a mouse eyeball snare is held with the index finger and thumb of the left hand, the annular structure of the mouse eyeball snare is pressed down from top to bottom right facing the upper surface of the eyelid of the mouse, the eyeball of the mouse is trapped and fixed by the annular structure after the eyelid of the mouse is pushed away by the annular structure, the eyeball of the mouse protrudes upwards out of the plane of the annular structure at the moment, and the annular structure is pressed downwards continuously till veins on the sclera of the eyeball of the mouse are exposed clearly.