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Cervical cancer prognosis test method

A detection method and technology for cervical cancer, which can be used in biochemical equipment and methods, measuring devices, and microbial determination/inspection, etc., and can solve problems such as tumor formation and malignant transformation of cells.

Active Publication Date: 2015-12-30
ZHEJIANG SUNSTEM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that low-level expression of NSD2 can promote development, and high-level expression or expression disorder can cause malignant transformation of cells and lead to tumor formation

Method used

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  • Cervical cancer prognosis test method
  • Cervical cancer prognosis test method
  • Cervical cancer prognosis test method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0086] Embodiment, the cervical cancer prognosis detection method, comprises the following steps in sequence:

[0087] A. Processing of tissue chips, including:

[0088] 1) Dewaxing and hydrating the obtained tissue chip, including:

[0089] Place the tissue chip at room temperature for 50, 60 or 70 minutes before dewaxing, or bake it in a 60°C incubator for 15, 20 or 25 minutes.

[0090] Afterwards, the tissue chips were soaked in xylene for 5, 10 or 15 minutes. Soak again for 5, 10 or 15 minutes after changing the xylene.

[0091] Afterwards, take out the tissue chips and soak them in absolute ethanol for 3, 5 or 8 minutes, in 90%, 93% or 95% ethanol for 3, 5 or 8 minutes, and in 70%, 72% or 75% ethanol. 3, 5 or 8 minutes.

[0092] 2) performing antigen thermal repair on the tissue chip formed in step 1), including:

[0093] Use a microwave oven to heat 0.01M sodium citrate buffer solution, the buffer solution has a pH value of 6.0, put the tissue chip into the buffer s...

experiment example

[0155] 1. Expression of SETD4 in cervical tumor cells

[0156] The expression level of SETD4 in normal cervical tissue, cervicitis tissue and cervical tumor tissue was detected by qRT-PCR. Quantitative PCR data showed that the expression level of SETD4 was low in normal cervical tissue. Compared with normal tissue, SETD4 in cervical inflammatory tissue was only slightly increased, but it was increased by 6 times in tumor tissue. See figure 1 As shown, quantitative PCR analyzed 10 samples in each group, and the results are shown in Table 1. Immunohistochemical examination found that only 4.5% of normal cervical tissues were positive for SETD4, 6.1% of cervicitis, and 76% of cervical tumor tissues. There were 6 specimens in each group for immunohistochemical examination.

[0157] Table 1: Positive rate of SETD4 detected by immunohistochemistry

[0158]

[0159] 2. The relationship between the positive level of SETD4 in cervical tumor cells and prognosis

[0160] The SETD4...

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Abstract

The invention discloses a cervical cancer prognosis test method which comprises the following steps sequentially of tissue chip processing, result judgment, determination and the like. According to the method, the SETD4 expression level is tested through immunohistochemistry and with a quantitative PCR method, a relatively normal tissue is found, the uterine cervicitis tissue SETD4 lightness is improved, the tumor tissue is improved remarkably, and the SETD4 expression level can be used for predicating cervical cancer prognosis and taken as a prognostic indicator for predicating the cervical cancer therapeutic effect.

Description

technical field [0001] The invention relates to the technical field of cervical cancer prognosis, in particular to a cervical cancer prognosis detection method. Background technique [0002] Worldwide, cervical cancer is the fourth most common malignant tumor in women, and its mortality rate ranks second, second only to breast cancer. According to data, in 2012, there were 528,000 cases of cervical cancer worldwide, and 266,000 deaths. Moreover, 85% of cervical cancers occur in developing countries, and cervical cancer ranks first among the causes of cancer death in these countries. In 2014, there were approximately 12,360 new cases of cervical cancer and 4,020 deaths in the United States. Although the incidence of cervical cancer in women in developed countries such as the United States is decreasing, cervical cancer remains a major worldwide health problem. [0003] The study of molecules with diagnostic and therapeutic significance in cervical cancer is the basis for m...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/573C12Q1/68
Inventor 李晨蔚
Owner ZHEJIANG SUNSTEM BIOTECH CO LTD
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