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Cerebrospinal fluid collection method

A collection method and cerebrospinal fluid technology, applied in medical science, veterinary equipment, inoculation and ovulation diagnosis, etc., can solve the problems of difficult animal maintenance, high technical difficulty, high requirements for technical personnel and materials, and achieve small trauma, simple operation, The effect of saving development time

Inactive Publication Date: 2016-02-24
WUXI APPTEC SUZHOU +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Cerebrospinal fluid collection or intrathecal injection is a commonly used technique in clinical practice. However, in current scientific research, cerebrospinal fluid collection or intrathecal injection in cynomolgus monkeys is generally carried out through subarachnoid catheterization. The requirements for materials and materials are relatively high, postoperative animal maintenance is difficult, and many facilities cannot meet the required conditions
At the same time, studies have shown that the spinal cannula will have a certain impact on the experimental results

Method used

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Examples

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Effect test

Embodiment 1

[0023] In the cerebrospinal fluid collection method of this embodiment, the collected object is the cerebrospinal fluid of a cynomolgus monkey (provided by Hainan Jingang Biotechnology Co., Ltd.) weighing 3-5 kg.

[0024] The required experimental materials are: anesthesia (ketamine or Zolta 50, lidocaine), povidone iodine, 75% alcohol cotton balls, dry cotton balls, sterile gloves, sterile gauze, 1.0mL-2.0mL syringe, 22G Syringe needles etc.

[0025] The operation steps of cynomolgus monkey cerebrospinal fluid collection are as follows:

[0026] Step 1, cynomolgus monkeys were anesthetized with 10-15 mg / kg ketamine (IM) or 4.4 mg / kg Sutai 50 (SC). Pay attention to monitor the anesthesia state of the animals during the operation. Animals should be fasted for at least 6 hours before anesthesia to avoid suffocation caused by food regurgitation during operation.

[0027] Step 2: According to the requirements of aseptic surgery, shave the lumbar region on the back of the animal...

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Abstract

The invention discloses a cerebrospinal fluid collection method. The method comprises the following steps: (1) carrying out animal anesthesia; (2) carrying out shaving and disinfection of a lumbar region of the back of an animal; (3) coating the puncture part of the animal with a wound towel; (4) carrying out puncture on the lumbar region of the back of the animal; (5) when a syringe needle punctures a subarachnoid space of the animal and generates a fall feeling, stopping inserting the syringe needle; (6) connecting an injector and collecting animal cerebrospinal fluid; and (7) pulling out the syringe needle after operation is ended, pressing the puncture part with a dry cotton ball, putting the animal back to a cage, and monitoring the animal until the animal is sober from anesthesia and the action is normal. The cerebrospinal fluid of the animal is collected through a lumbar puncture method; and the cerebrospinal fluid collection method is small in wound to the animal, simple and fast to operate and low in demands on equipment, materials and places.

Description

technical field [0001] The invention relates to the biological field, in particular to a method for collecting cerebrospinal fluid. Background technique [0002] Cerebrospinal fluid collection or intrathecal injection is a commonly used technique in clinical practice. However, in current scientific research, cerebrospinal fluid collection or intrathecal injection in cynomolgus monkeys is generally carried out through subarachnoid catheterization. The requirements for materials and materials are relatively high, postoperative animal maintenance is difficult, and many facilities cannot meet the required conditions. At the same time, studies have shown that the spinal cannula will have a certain impact on the experimental results. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a method for collecting cerebrospinal fluid, which is simple in operation and low in cost. [0004] In order to solve the above-mentioned ...

Claims

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Application Information

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IPC IPC(8): A61B10/00A61D7/00
CPCA61B10/0045A61B2010/0077A61B2503/40A61D7/00
Inventor 薛彬金毅周聪颖王福强张伟军胡璇毕玲玲谢瑞东王新峰赵莹
Owner WUXI APPTEC SUZHOU
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