Depth of field-increasing microscopic optical system

Abstract

The invention discloses a depth of field-increasing microscopic optical system comprising a sample stage used for placing samples, a lighting system and a light source which are disposed below the sample stage, a microscopic objective disposed above the sample stage, a tube lens disposed above the microscopic objective, a splitting prism disposed between the microscopic objective and the tube lens or above the tube lens, and at least two imaging elements disposed on the imaging side of the splitting prism, wherein the imaging elements differ in back focal length. According to the invention, the splitting prism is added on the basis of a traditional microscopic optical system, the location of a taken object plant is changed by changing the back focal length of the optical system, and therefore, object planes taken by different imaging elements are in different positions. Under the condition of not affecting the imaging quality, the depth of field is increased, and a high-resolution image of a living body sample or fluid sample can be taken in real time.

Description

technical field [0001] The invention relates to the field of optical technology, in particular to a microscopic optical system for increasing the depth of field. Background technique [0002] In the process of continuous development of the optical microscope, its function is becoming more and more powerful, but because in the microscopic optical system, the higher the magnification of the microscopic objective lens, the larger the numerical aperture, the smaller the depth of field, and the tiny defocus will be Therefore, in the practical application of the microscope, it is of great significance to expand the depth of field range of the imaging system while maintaining the imaging quality of the imaging system. In today's microscopic imaging field, more studies are being carried out to improve the depth of field of the microscopic system through scanning imaging. However, the scanning imaging method requires the system to consume a lot of time in scanning calculations, and ...

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Problems solved by technology

[0004] In view of the above deficiencies in the prior art, the object of the present invention is to provide a microscopic optical system that increases the depth of field, aiming at solving the problem that the existing microscopic optical system cannot simultaneously ensure the imaging quality and expand the range of the depth of field

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