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Cervical cancer detection polypeptide and detection device and detection kit

A detection kit, cervical cancer technology, applied in biological tests, peptides, instruments, etc., can solve the problems of low sensitivity and poor specificity

Inactive Publication Date: 2016-03-09
SUZHOU SJ BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently reported autoantibody detection methods have low sensitivity and poor specificity, and the false negative rate can be as high as 50%.

Method used

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  • Cervical cancer detection polypeptide and detection device and detection kit
  • Cervical cancer detection polypeptide and detection device and detection kit
  • Cervical cancer detection polypeptide and detection device and detection kit

Examples

Experimental program
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Embodiment

[0063] Hereinafter, the present invention will be described more specifically through examples, but the technical scope of the present invention is not limited. Through the description of this specification, those skilled in the art can easily modify / change the present invention, and these are included in the technical scope of the present invention. The scope of the invention is determined by the claims.

[0064] 1. Peptide preparation and confirmation

[0065] The polypeptide used in the examples has the amino acid sequence shown in SEQ ID NO: 1, and was synthesized by Chengdu Capgemini Biomedical Technology Development Co., Ltd. The characterization of the polypeptide can confirm that the polypeptide was synthesized by hydrogen spectrum and mass spectrum. Liquid chromatography detection purity: 95.66% (area normalization method): mass spectrometry detection (ESI-MS): calculated molecular weight: 2154.57, test value 2153.4.

[0066] 2. Fabrication of Detection Devices

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PUM

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Abstract

The present invention belongs to the technical field of immunoassay, and relates to a cervical cancer early diagnosis polypeptide composition and a detection device and a detection kit, the detection kit further comprises the detection device, the detection device comprises one or more solid carriers and the specific polypeptide composition independently connected with the more solid carriers, and the detection device and the detection kit can be used for early diagnosis and screening of cervical cancer.

Description

technical field [0001] The invention belongs to the field of biological detection, in particular to a polypeptide that can be used for early screening and clinical auxiliary diagnosis of cervical cancer, and a detection kit for a detection device containing the polypeptide. Background technique [0002] Antigen-antibody detection is a common detection method for immunoassay. A large number of studies have shown that tumor-associated antigens in serum or plasma can induce the body to produce autoantibodies, and there are both tumor antigens and white antibodies against the tumor antigens in the serum of many patients. Therefore, both antibodies can be used to detect tumor antigens, and antigens can also be used to detect tumor antigen autoantibodies, but the specificity and sensitivity of using tumor autoantibodies to detect tumors are much higher than using tumor antigens to detect tumors. Many tumor-associated antigens exist not only in tumor patients, but also in normal p...

Claims

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Application Information

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IPC IPC(8): C07K7/08G01N33/68G01N33/574G01N33/545
CPCC07K7/08G01N33/545G01N33/57411G01N33/68G01N2410/00
Inventor 马宏伟忻寅强马雄明
Owner SUZHOU SJ BIOMATERIALS
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