Identification method of loquat SRAP marking system and loquat dwarf hybrid variety

Abstract

The invention relates to the field of agricultural application, in particular to an identification method of a loquat SRAP (Sequence-Related Amplified Polymorphism) marking system and a loquat dwarf hybrid variety. The method comprises the steps that optimization is conducted on the annealing temperature in an SRAP amplification program and the content of four main factors of DNA, dNTPs, a Taq enzyme and Primers in an amplification system, and on the basis, SRAP marking identification and analysis are conducted on the authenticity of a loquat dwarf hybrid variety 861 strain and genetic far and near relationships between the loquat dwarf hybrid variety 861 strain and eight white flesh loquat varieties. According to the identification method of the loquat SRAP marking system and the loquat dwarf hybrid variety, it is indicated that the optimized SRAP system can be well applied to loquat molecular assistant breeding work such as loquat hybrid variety identification, genetic relationship classification and the like.

Description

technical field [0001] The invention relates to the field of agricultural applications, in particular to a loquat SRAP marking system and an identification method for loquat dwarf hybrids. Background technique [0002] Loquat (Eriobotrya japonica Lindl.) is an evergreen arbor fruit tree belonging to the Rosaceae apple subfamily (Mloideae) and the genus Loquat (Eriobotrya). The fruit is rich in nutrients and has medicinal value such as cough relief and anticancer, and its cultivation has high economic value. my country is the origin of loquat, with abundant germplasm resources. There are 21 major species of Loquat plants native to my country, and more than 100 cultivars of loquat have been cultivated in my country. However, at present, my country's loquat cultivars are still dominated by large or medium-crown arborization and sparsely planted trees. There is a lack of dwarf and densely planted varieties, which will easily lead to inconvenient management, reduced yield, qualit...

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Problems solved by technology

[0004] The optimal response system of SRAP is related to the species of plants. The predecessors optimized the SRAP system on apples, grapes, sanhua plums, oranges, persimmons, macadamia nuts, walnuts and other fruit trees. Qiao Yanchun et al. [1

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