Application of TPO (thrombopoietin) receptor agonist in promoting homing of HSCs (hematopoietic stem cells)
A thrombopoietin and receptor agonist technology, which is applied in the field of promoting hematopoietic stem cell homing and thrombopoietin receptor agonist, can solve the problems that the drug needs further research and achieve the effect of improving the homing efficiency
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[0038] The effect of the thrombopoietin receptor agonist eltrombopag on hematopoietic stem cell homing after bone marrow cell transplantation was examined as follows:
[0039] 1. Isolation of mouse bone marrow cells
[0040] C57BL / 45.1 strain mice were purchased from Beijing Huafukang Biotechnology Co., Ltd., 6-8 weeks old, 18-22g body weight, male, and these mice were used as bone marrow cell transplantation donors. The early hematopoietic stem progenitor cells and leukocytes of this mouse stably express CD45.1, and are used in competitive transplantation experiments together with C57BL / 45.2 strain mice. The mice were in a stable state for more than one week, and then sacrificed by spinal dislocation. The femur and tibia were taken out, and the bone marrow cavity was washed repeatedly with PBS. TM LysingSolution, CAT.No: 55899, dilute with deionized water 1:10 before use), add an equal volume of PBS after 2 minutes, centrifuge at 600g for 5 minutes, and wash twice to obtain ...
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