Method for quickly and quantitatively evaluating blood vessel generation promotion function of chemical compounds on zebra fish

Abstract

The invention relates to a method for quickly and quantitatively evaluating a blood vessel generation promotion function of chemical compounds on zebra fish. According to the method, transgenic zebra fish with blood vessels specially expressing green fluorescent protein are adopted, a to-be-detected medicine is directly added to water for culturing the zebra fish, the relative area of tail venous rete of the zebra fish is detected, and the method for quickly and quantitatively evaluating the blood vessel generation promotion function of the chemical compounds on the zebra fish is established and used for medicine screening. In the experiments of the zebra fish, the relative area of the tail venous rete is initially taken as a new evaluation index about the blood vessel generation function, whether the to-be-detected medicine has a blood vessel generation promotion function is analyzed more scientifically and more objectively, and the accuracy of results is improved.

Description

technical field [0001] The present invention relates to a method for rapidly and quantitatively evaluating the angiogenesis-promoting effect of compounds on zebrafish, specifically a method for rapidly evaluating exogenous drugs or The method for promoting the effect of environmental foreign matter on zebrafish angiogenesis belongs to the field of drug screening. Background technique [0002] Angiogenesis is associated with a variety of diseases. Angiogenesis not only exists during embryonic development, but also has important physiological and pathological significance throughout the life cycle. In adults, it is involved in many pathological processes such as tumors, inflammation, ischemic diseases, diabetic retinopathy, and wound healing, and has become the focus of research. Angiogenesis is a continuous and complex process, which is the result of synergy between cells (endothelial cells, pericytes, smooth muscle cells, fibroblasts) and between cells and cytokines in dif...

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Problems solved by technology

2. In the method using the inferior intestinal vein as an indicator, it is very difficult to take pictures of the inferior intestinal vein itself because it has a basketball mesh structure. The position of the zebrafish during shooting seriously affects the imaging of the inferior intestinal vein, which in turn affects the calculation and statistics of the results

[0006] Chinese patent document CN101179935 discloses a kind of angiogenesis of ischemic retinal tissue and a screening method for this purpose. The screening method uses mice as a model, and administers potential therapeutic agents to the eyes of the mice; Mice were euthanized and substantially the entire retina was removed from the eye administered the putative therapeutic agent; the retinal vasculature of the eye administered the putative therapeutic agent was stained and microscopic images were prepared showing the The area of ​​vascular occlusion and the area of ​​preretinal neovascular plexus in the stained retina; at the same time, a control experiment was performed, and the area of ​​preretinal neovascular plexus in the stained retina was compared with the preretinal blood vessels that could be observed in the stained retina of the mouse control eye In this method, the effect of the tested drug is limited by the effect of angiogenesis inhibitors. The tested drug reverses the target and pathway of the inhibitor used. The scope of screening is limited by the selection of the inhibitor, and cumbersome steps

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