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Application of autophagy-related protein 12 in the prevention and treatment of enterovirus 71 infection

A technology of autophagy-related protein and enterovirus, applied in the field of biomedicine

Active Publication Date: 2019-02-26
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the role of Atg12 and its expression product ATG12 in the process of EV71 infection has not been reported yet.
[0008] At present, there is no report on the role of ATG12 molecule in EV71 infection of HBMEC. In-depth research on this molecule can not only improve the understanding of EV71 infection and pathogenic mechanism, but also provide new ideas and targets for the prevention and treatment of EV71 infection

Method used

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  • Application of autophagy-related protein 12 in the prevention and treatment of enterovirus 71 infection
  • Application of autophagy-related protein 12 in the prevention and treatment of enterovirus 71 infection
  • Application of autophagy-related protein 12 in the prevention and treatment of enterovirus 71 infection

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Experimental program
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Embodiment 1

[0049] 1. Design and synthesize specific siRNA sequences for each host cell molecule.

[0050] 1.1 For each target gene, search NCBI GeneBank to obtain the full sequence and mRNA sequence, use existing network resources and common software to carry out biological analysis of each target gene, and select the coding region as the target sequence for siRNA design. Refer to the principle of siRNA design and compare it with the human genome sequence through the blast function of the GeneBank database to ensure no homology; exclude potential siRNAs that pair with other genes with 8 consecutive bases at the 5' end of the aitisense chain; exclude any continuous 14 nucleotides potential siRNAs for base pairing with other genes. The design software was used for pre-assessment and determination, and 3 optimal kinetic parameter targets were selected to enter the subsequent experimental process. A total of 3 interference sequences were synthesized for each gene, as shown in Table 1.

[00...

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Abstract

The invention belongs to the technical field of biomedicine, and relates to a novel target used for preventing enterovirus 71 infection, and applications thereof. According to the invention, Human Brain Microvascular Endothelial Cell (HBMEC) is taken as the target cell, and RNA interference technology is adopted for down-regulation of expression of target cell host protein so as to search host factors capable of inhibiting EV71 infection of HBMEC effectively, protect the functions of the blood brain barrier, and prevent central nervous system infection caused by penetrating of the blood brain barrier by virus. It is fond by experiments that the autophagy-related protein 12 (ATG12) possesses significant importance in infection of HBMEC by EV71, and down-regulation of expression of ATG12, and is capable of inhibiting EV71 infection obviously. The invention also provides applications of ATG12 in preparing drugs used for preventing or treating EV71 infection.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and is a new target and application of anti-enterovirus 71 infection. Background technique [0002] Enterovirus 71 (EV71) belongs to enteroviruses of the Picornaviridae family and belongs to human enterotype A virus, and is one of the main pathogens causing hand-foot-mouth disease (HFMD). At present, hand, foot and mouth disease outbreaks and epidemics in many parts of the world, especially in the Asia-Pacific region. In my country, since the outbreak of hand, foot and mouth disease in several major provinces and cities in 2008, the number of infections and the mortality rate of the disease have remained high, with more than 1 million cases reported each year and nearly 1,000 deaths. Hand, foot and mouth disease mainly affects infants and young children under the age of 5. The clinical manifestations are fever, and symptoms such as herpes appear on the hands, feet, buttocks, and oral mucosa....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61P31/14
CPCA61K48/00
Inventor 杨小康朱勇喆徐庆强朱耐伟戚中田宋洪元
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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