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Placenta preservation method, placenta preservation fluid and placenta preservation fluid preparing method

A preservation method and preservation solution technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve problems such as inconvenient application, cumbersome preparation process, and complex components of the preservation solution, and achieve good proliferation speed and preparation Simple steps, no toxic side effects

Active Publication Date: 2016-04-06
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the composition of the preservation solution is complicated, and the preparation process is cumbersome, so it cannot be applied conveniently.

Method used

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  • Placenta preservation method, placenta preservation fluid and placenta preservation fluid preparing method
  • Placenta preservation method, placenta preservation fluid and placenta preservation fluid preparing method
  • Placenta preservation method, placenta preservation fluid and placenta preservation fluid preparing method

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preparation example Construction

[0030] The preparation method of umbilical cord plasma is as follows: transfer the umbilical cord blood into a 50mL centrifuge tube, seal and centrifuge at 2500rpm for 15min, absorb the upper light yellow plasma into a new centrifuge tube, and store it at 4°C for later use.

[0031] When preparing the placenta preservation solution of the present invention, low molecular dextran glucose injection (purchased from Guangzhou Pharmaceutical Company) was directly used for low molecular dextran-40, which contained low molecular dextran-40 at a concentration of 6m / v%, containing glucose The concentration is 5 m / v%.

Embodiment 1

[0032] Embodiment 1, placenta preservation solution

[0033] Add appropriate amount of umbilical cord blood plasma, 20m / v% glucose solution, 6m / v% low molecular weight dextran glucose injection and 100× penicillin-streptomycin to PBS, after mixing, the contents of each component in the placental preservation solution are as follows: 5v / v% umbilical cord plasma, 5m / v% glucose, 1× penicillin-streptomycin, 1m / v% low molecular weight dextran-40, PBS balance.

Embodiment 2

[0034] Embodiment 2, placenta preservation solution

[0035] Add an appropriate amount of umbilical cord blood plasma, 20m / v% glucose solution, 6m / v% low molecular weight dextran glucose injection and 100× penicillin-streptomycin to PBS, and after mixing, the contents of each component in the placental preservation solution are as follows: 3v / v% umbilical cord plasma, 5m / v% glucose, 1× penicillin-streptomycin, 1m / v% low molecular weight dextran-40, PBS balance.

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Abstract

The invention discloses a placenta preservation method, placenta preservation fluid and a placenta preservation fluid preparing method. The placenta preservation fluid contains cord plasma and / or glucose, penicillin-streptomycin and low molecular dextran-40. The placenta preservation fluid preparing method comprises the step of adding 3-5 v / v% of cord plasma, 5 m / v% of glucose solution, 1X-3X of penicillin-streptomycin and low molecular dextran glucose injection to PBS, wherein the final concentration of low molecular dextran-40 is 1 m / v%. According to the placenta preservation method, placentas are soaked in the placenta preservation fluid to be preserved in a dark place at 2-4 DEG C. An ion buffer system and a nutrition system of the preservation fluid are improved, constituents and content of constituents in the preparation are optimized, long-time preservation of in vitro placentas can be achieved, the vigor of placental mesenchymal stem cells can be well maintained, and functional variation of cells is avoided.

Description

technical field [0001] The invention relates to the field of preservation of biological isolated tissues or organs, in particular to a placenta preservation method, a placenta preservation solution and a preparation method thereof. Background technique [0002] Mesenchymal stem cells are a type of cells with self-replication and multi-lineage differentiation capabilities. They can continuously self-renew and transform into one or more cells that constitute human tissues or organs under specific conditions. Studies have found that mesenchymal stem cells are mainly derived from bone marrow, umbilical cord blood, umbilical cord tissue, placental tissue, and adipose tissue. Compared with other stem cells, placental mesenchymal stem cells have the advantages of convenient source, sufficient number of cells, and easy isolation, culture, expansion and purification. After more than 30 passages, they still have the characteristics of stem cells, and are currently the best proven stem...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 陈海佳王一飞葛啸虎卢瑞珊王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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