Pig-specific friendly site pifs102 and its application

Abstract

The invention relates to the field of genetic engineering and particularly discloses a pig specific friendly site Pifs102. The position of the friendly site Pifs102 in a genome is 98768519bp on the chromosome 13 (NC_010455.4, based on the pig genome sequence information version number Sscrofa10.2, September, 2011). The pig specific friendly site Pifs102 can be used for stable expression of exogenous genes, and the problems of instable expression of the exogenous genes and unpredictability of the position effect during transgenosis are solved. By means of the pig specific friendly site Pifs102, fixed-site exogenous gene integration to livestock becomes reliable, and the foundation for fixed-site integration of the livestock is laid. A targeting vector provided on account of the pig specific friendly site Pifs102 can achieve targeting efficiently and specifically.

Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular to the pig-specific friendly site Pifs102. Background technique

[0002] In the production process of transgenic animals, whether foreign genes can be expressed efficiently and stably in transgenic animals is the key to transgenic technology. The exogenous gene is randomly integrated in the genome of the recipient animal, and the expression level will be unstable due to the position effect, and the transgene will be silenced by the epigenetic modification. In order to achieve sustained and stable expression of exogenous genes, site-specific transgenesis can solve various problems caused by position effects. In recent years, ZFN and TALEN technologies, as well as the emerging CRISPR / Cas9 technology, have provided new tools for site-specific integration and gene knockout of genes, improved the efficiency of transgenics, and have been successful in many species. However, one of the...

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