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High-cost-performance human blood immune globulin G detection reagent kit

A technology of immunoglobulin and detection kit, which is applied in the field of latex immunoturbidimetric human blood immunoglobulin G detection kit, which can solve the problem that the performance of the reagent cannot compete with BC, and avoid underestimation of clinical results or even false negative results , Extensive range of antigen excess, and the effect of saving patients' expenses

Inactive Publication Date: 2016-05-11
BYRON DIAGNOSTICS SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology helps identify specific proteins called lgG by measuring their concentration levels in serum from patients who have been infected (or are at risk) while they wait until symptoms appear. By comparing these measurements against existing standards, we aim to improve our ability to diagnose diseases caused by viruses such as HIV-1 virus.

Problems solved by technology

This patented describes different methods used by physicians when diagnosing or treating certain medical conditions that may cause inflammation called Allergy Asthma Inflammatory Boweldanitis Syndromes (Asthma). These symptoms include high levels of circulating immunosuppressive proteins like interleukin-4-gamma (IL-4), which help regulate blood cell production. However, these tests only detect one protein at once per day, making it difficult if there's another substance involved in causing them. Therefore, researchers often look into other sources where they could find out whether any new drugs were effective against AI asthmatic attacks caused by specific viruses or bacteria found during previous epidemics.

Method used

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  • High-cost-performance human blood immune globulin G detection reagent kit
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  • High-cost-performance human blood immune globulin G detection reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The particle size of the latex particles in this example is 90nm, and 25ml of the rabbit anti-human immunoglobulin G polyclonal antibody produced by Dako Company of Denmark (article number: Q0331) is labeled per liter of reagent 2, and the most common phosphate buffer in latex immunoturbidimetry is used. As reagent 1, the most common parameters of the automatic analyzer are reagent 1: reagent 2 ratio 180 μl: 60 μl, the sample is pre-diluted 80 times and then 3 μl of the diluted sample is added to the reaction system (that is, the ratio of sample to antibody is 1:40).

[0037] Using Hitachi7170 automatic biochemical analyzer, the wavelength is 600nm single wavelength, and the two-point endpoint method is calculated at 18-34 reading points. The data are as follows:

[0038] Table 1 Calibration data

[0039] Concentration of calibrator ㎎ / L 0 5 10 20 40 60 Absorbance change value 10 992 1917 3897 7344 10332

[0040] Table 2 Linear Dilution

...

Embodiment 2

[0049] The particle size of the latex particles in this example is 40nm, and 20ml of the rabbit anti-human immunoglobulin G polyclonal antibody produced by Dako Company of Denmark (article number: Q0331) is labeled per liter of reagent 2, and the most common phosphate buffer in latex immunoturbidimetry is used. As reagent 1, the most common parameters of the automatic analyzer are reagent 1: reagent 2 ratio 180 μl: 60 μl, the sample is pre-diluted 80 times and then 3 μl of the diluted sample is added to the reaction system (that is, the ratio of sample to antibody is 1:32).

[0050] Hitachi7170 automatic biochemical analyzer was used, the wavelength was 600nm single wavelength, and the two-point endpoint method was used to calculate 18-34 reading points. The data are as follows.

[0051] Table 5 Calibration data

[0052] Concentration of calibrator ㎎ / L 0 5 10 20 40 60 Absorbance change value 8 284 589 1239 2345 3121

[0053] Table 6 Linear Dilut...

Embodiment 3

[0062] The particle size of the latex particles in this example is 90nm, and 20ml of the rabbit anti-human immunoglobulin G polyclonal antibody produced by Dako Company of Denmark (article number: Q0331) is labeled per liter of reagent 2, and the most common phosphate buffer in latex immunoturbidimetry is used. As reagent 1, the most common parameters of the automatic analyzer are reagent 1: reagent 2 ratio 180 μl: 60 μl, the sample is pre-diluted 80 times and then 3 μl of the diluted sample is added to the reaction system (that is, the ratio of sample to antibody is 1:32).

[0063] Hitachi7170 automatic biochemical analyzer was used, the wavelength was 600nm single wavelength, and the two-point endpoint method was used to calculate 18-34 reading points. The data are as follows.

[0064] Table 9 Calibration data

[0065] Concentration of calibrator ㎎ / L 0 5 10 20 40 60 Absorbance change value 10 699 1311 2511 4577 6123

[0066] Table 10 Linear Di...

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Abstract

The invention discloses a high-cost-performance human blood immune globulin G detection reagent knit and a preparation method thereof. The reagent kit comprises a reagent 2. The reagent 2 is the latex particle solution marked with a rabbit antihuman immune globulin G polyclonal antibody, wherein the latex particle size range is 40-120 nm, and the consumption of the antibody in per liter of the reagent 2 is 20-30 ml. The high-cost-performance human blood immune globulin G detection reagent knit can replace an imported reagent, disease cost is saved, and meanwhile the detection efficiency can be improved.

Description

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Claims

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Application Information

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Owner BYRON DIAGNOSTICS SHANGHAI
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