A qualitative and quantitative detection method of intestinal microorganisms for non-diagnostic purposes

Abstract

The invention discloses a method for qualitative and quantitative testing of intestinal microorganisms and belongs to the technical field of biology. The method comprises the steps that a target microorganism class group, target microorganisms and non-target organisms in a sample to be tested and reference microorganisms not existing in the sample to be tested are determined; a feature area for the target microorganism class group and the target microorganisms is designed; multiple amplification primers of the feature area are designed; after the reference microorganisms and exogenous nucleic acid are added into the sample to be tested, nucleic acid of the microorganisms in the sample to be tested is extracted; the designed multiple primers are utilized for amplifying the nucleic acid of the microorganisms, and a feature sequencing segment is obtained after amplification; the feature sequencing segment is utilized for qualitatively and quantitatively analyzing the microorganisms in the sample to be tested. According to the method, there is no need to perform preculture or proliferation on the microorganisms, multiple known microorganisms in the sample to be tested can be tested at one time at high flux, accuracy and resolution, the testing process is simple and quick, and the procedures are standard.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a qualitative and quantitative detection method for non-diagnostic intestinal microorganisms. Background technique [0002] Intestinal microbes are an important basis for the diagnosis of intestinal diseases, and accurate qualitative and quantitative detection of intestinal microbes is very necessary. [0003] Existing qualitative and quantitative detection technologies for intestinal microorganisms include morphological counting, chip detection, 16S rRNA sequencing, metagenomic sequencing, and real-time quantitative PCR (Polymerase Chain Reaction, polymerase chain reaction). Morphological counting detection requires pre-cultivation of microorganisms, which takes a long time, non-culturable microorganisms cannot be detected, and only one type of microorganism can be detected at a time, the throughput is low, the sampling amount is limited when counting, and the results are rough, and...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem of inaccurate qualitative and quantitative detection of microorganisms in the prior art, the embodiment of the present invention provides a qualitative and quantitative detection method of intestinal microorganisms for non-diagnostic purposes

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