Preparation method of detection card for direct antiglobulin test

Abstract

The invention relates to a preparation method of a detection card for a direct antiglobulin test. The detection card comprises eight microcolumn gel tubes, including Coombs1, Coombs2, IgG1, IgG2, C3d1, C3d2, K1 and K2, wherein the Coombs1 and the Coombs2 microcolumn gel tubes are separately filled with multispecific antiglobulin gel of equal quantities, the IgG1 and the IgG2 microcolumn gel tubes are separately filled with anti-IgG gel of equal quantities, the C3d1 and the C3d2 microcolumn gel tubes are separately filled with anti-C3d gel of equal quantities, and the K1 and the K2 microcolumn gel tubes are separately filled with blank gel of equal quantities. The preparation method comprises the following steps: preparation of glucan gel particles; preparation of each gel washing buffer; selection of each antibody; washing of gel; split charging, etc.; and the detection card for the direct antiglobulin test is formed. The prepared detection card for the direct antiglobulin test has the advantages of high sensitivity, good specificity, strong stability, long shelf-life, complete detection items, etc.

Description

technical field [0001] The invention relates to the field of blood transfusion medicine, in particular to a preparation method of a direct antiglobulin detection card. Background technique [0002] Direct antiglobulin test is to check whether there are incomplete antibodies and complement sensitization on the tested red blood cells, also known as Coombs test. Direct antiglobulin test is an important basis for the diagnosis of hemolytic disease of the newborn (HDN), immune hemolytic anemia (AIHA), immune hemolytic transfusion reaction, and drug-induced immune hemolytic anemia. [0003] Micro-column gel immunoassay technology is an immunoassay technology produced in the 1990s. Its basic principle is to use molecular sieve technology, centrifugal technology and specific immune reaction technology to combine blood group serology technology with gel molecular sieve technology. It can sensitively detect the possible weak blood group antigen antibody reaction, which is considered ...

AI Technical Summary

Problems solved by technology

[0004] At present, some manufacturers at home and abroad have produced direct anti-human globulin detection cards, but there are some shortcomings: one is the use of a single type of dextran gel or polypropylene dextran gel, which makes it difficult to balance sensitivity and specificity; The second is to use normal saline or antibody buffer to swell the gel, so that the gel cannot be fully swollen and the ionic strength of the reaction system is destroyed; the third is that the gel washing buffer is exactly the same for the gel with different antibodies, which cannot effectively protect the gel. Antibody stability; the fourth is that the test items are incomplete, some do not contain polyspecific anti-human globulin reagents, and the sensitization of red blood cells cannot be fully detected; some do not contain red blood cells themselves. Autoagglutination caused by interfering with results