Method for screening specific serum metabolism markers for triple-negative breast cancer

A triple-negative breast cancer and metabolic marker technology, applied in the field of biomarkers, can solve the problem that there is no biomarker for screening the metabolic changes of triple-negative breast cancer, and achieve the effect of high sensitivity

Active Publication Date: 2016-07-06
重庆韦钚医药科技有限公司
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Problems solved by technology

[0003] However, in the prior art, there is no method for screening biomarkers of body metabolic changes caused by triple-negative breast cancer

Method used

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  • Method for screening specific serum metabolism markers for triple-negative breast cancer
  • Method for screening specific serum metabolism markers for triple-negative breast cancer
  • Method for screening specific serum metabolism markers for triple-negative breast cancer

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Embodiment Construction

[0030] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0031] A method for screening triple-negative breast cancer-specific serum metabolic markers, the method comprising the steps of:

[0032] (1) A number of sera from female patients with triple-negative breast cancer were selected as the experimental group A; normal female sera equal to the number of the experimental group A were selected as the control group B. In this example, the serum of 31 female patients with triple-negative breast cancer was analyzed, and the serum of 31 normal females was used as a control.

[0033] (2) After thawing the serum of experimental group A and control group B at room temperature, accurately pipette 100 μL of serum samples into different 1.5mLEP tubes, use methanol to precipitate protein, and the ratio is sample:methanol=1:4, vortex for 30s, Centrifuge at 12,000 rpm for 15 minutes at 4°C, draw 200 μL...

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Abstract

The invention discloses a method for screening specific serum metabolism markers for triple-negative breast cancer. The method includes respectively carrying out metabonomics analysis on serum samples of experimental groups A and control groups B by the aid of LC / MS (liquid chromatography / mass spectrometry) instruments; carrying out model discriminant analysis on response intensity data of peaks of substances in the samples; respectively carrying out PCA (principal component analysis) on the experimental groups A and the control groups B; building PLS-DA (partial least square- discriminant analysis) and OPLS-DA (orthogonal partial least square-discriminant analysis) models on the basis so as to obtain difference expression metabolites; screening and identifying the biological markers related to breast cancer carcinogenesis and metastasis. The biological markers include hemolytic lecithin, sphingomyelin and small-molecule amino acid. The method has the advantages that results obtained by the aid of the method have an important significance on illustrating change rules of contents of characteristic metabolites in the serum of patients who suffer from the triple-negative breast cancer and illustrating effects of the metabolites in tumor formation and development procedures; effective breast cancer early diagnosis target sites can be acquired by the aid of the method, and data bases can be provided for establishing specific cancer diagnosis models.

Description

technical field [0001] The present invention relates to a metabonomics method for overall evaluation of body metabolic changes caused by tumors and screening of valuable biomarkers, in particular to a method for screening specific serum metabolic markers of triple-negative breast cancer. Background technique [0002] Metabolomics is a technology developed in recent years, which is used to evaluate the changes of various small molecular compounds in biological samples under the stimulation of the environment or other factors. Early detection, early treatment, and personalized diagnosis and treatment of tumors are the development direction of tumor treatment. The emerging metabolomics has opened up new ideas for assisting early diagnosis, efficacy evaluation, and prognosis of tumors. The occurrence and development of tumors are accompanied by changes in the body's metabolites. Metabolomics can make an overall evaluation of the body's metabolic changes caused by tumors, and scr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027G01N2570/00
Inventor 辇伟奇李丽仙郑晓东易琳张海伟林昌海刘兴明
Owner 重庆韦钚医药科技有限公司
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