Screening method for specific serum markers of hepatitis B

A technology for hepatitis B and screening method, applied in the field of medicine and biology, can solve the problems of long waiting time for test results, high cost of reagents, complex test methods and the like

Active Publication Date: 2021-06-11
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] HBV and other blood transfusion-related pathogens have an important impact on the blood safety of our country. For blood donors, on-site HBV related tests cannot be done. Only after the blood donation is completed, the five routine hepatitis B tests and HBV nucleic acid tests are concentrated. The disadvantage is that it takes a long time to wait for the test results, the detection method is complicated, and the reagent cost is high

Method used

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  • Screening method for specific serum markers of hepatitis B
  • Screening method for specific serum markers of hepatitis B
  • Screening method for specific serum markers of hepatitis B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0153] Example 1, Method for screening metabolic markers in blood of hepatitis B virus infected

[0154] I. Take 30 cases of hepatitis B patients (HBG), diagnosis of standard HBV nucleic acid detection positive or HBV enzyme free two or three positive, eliminating any of liver cancer and other infection or disease, early morning serum, 1 hour Saved -80 ° C, and 30 cases of the same age health checkchard (CON), and stored in -80 ° C in 1 hour. After collecting, the following methods are unified.

[0155] Second, reverse phase chromatography analysis serum sample processing method

[0156] 1) Plasma / serum sample stored at a negative 80 ° C for 30-60 minutes of ice melting at 4 ° C.

[0157] 2) Take 100 ul serum to a 1.5 ml centrifuge tube marked, add 600 ul, volume ratio of 3: 1 chlorine imitation: mixed solution of methanol, ultrasonic 1H, add 100 ul, mix.

[0158] 3) 12000 rpm, 4 degrees Celsius, centrifugation for 10 minutes, remove the chloroform 300 uL to concentrate dry, add...

Embodiment 2

[0189] The targeting detection of metabolic markers in a group of patients with a group of viral hepatitis, mainly includes the following steps:

[0190] 1. Take 70 cases of hepatitis B patients (HBV), diagnostic standard HBV nucleic acid detection positive or HBV enzyme free two or three positive, eliminating any of liver cancer and other infection or disease, early morning blood, 1 hour Saved -80 ° C, and 30 cases of the same age health checkchard (CON), and stored in -80 ° C in 1 hour. After collecting, the following methods are unified.

[0191] 2, reverse phase chromatography analysis serum sample processing method

[0192] 1) Plasma / serum sample stored at -80 ° C for 30-60 minutes of ice melting at 4 ° C.

[0193] 2) Take 100 ul serum to the 1.5ml centrifuge tube of the marked tag, add 600 ul chloroform: methanol 3: 1, ultrasonic 1h, add 100 ul, mix.

[0194] 3) 12000 rpm, 4 degrees Celsius, centrifugation for 10 minutes, remove the chloroform 300 uL to concentrate dry, ad...

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Abstract

The invention belongs to the technical field of medicine and biology, and relates to a screening method for heterosexual serum markers in patients, in particular to a screening method for hepatitis B specific serum markers. The screening method of the present invention includes the following steps: collection and storage of serum samples, processing method of serum samples, technical conditions of normal phase and reverse phase chromatography, mass spectrometry data collection and analysis, non-targeted metabolome data processing, significant difference Screening of results, verification and application of screening results.

Description

Technical field [0001] The present invention belongs to the field of medical biotechnology, and the screening method involving the in vivo heterogeneous serum marker, specifically a screening method of hepatitis B-specific serum marker. Background technique [0002] The pathogen of hepatitis B is hepatitis B virus, abbreviated as HBV, and hepatitis B virus is a DNA virus. B strain viral hepatitis is a infectious disease mainly caused by hepatitis B virus. Clinically, degrade, nausea, upper abdomen, and weakness in the liver district pain, and fatigue. Some patients may have jaundice fever and liver allocate with liver function damage. Some patients can be slow, and even developed into liver cirrhosis, and a small number can be developed into liver cancer. [0003] my country is a high hepatitis B virus infection, before intervention measures such as hepatitis B vaccine vaccination and blood screening, the population of hepatitis B virus surface antigen (HBSAG) is about 10%. [00...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N33/576
CPCG01N30/88G01N33/5761G01N2030/8813
Inventor 张婷杨帆
Owner INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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