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Screening method for hepatitis B specific serum marker

A hepatitis B, screening method technology, applied in the field of medicine and biology, can solve the problems of high cost of reagents, long waiting time for test results, complicated detection methods, etc.

Active Publication Date: 2019-07-26
INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] HBV and other blood transfusion-related pathogens have an important impact on the blood safety of our country. For blood donors, on-site HBV related tests cannot be done. Only after the blood donation is completed, the five routine hepatitis B tests and HBV nucleic acid tests are concentrated. The disadvantage is that it takes a long time to wait for the test results, the detection method is complicated, and the reagent cost is high

Method used

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  • Screening method for hepatitis B specific serum marker
  • Screening method for hepatitis B specific serum marker
  • Screening method for hepatitis B specific serum marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0154] Example 1. Method for Screening Metabolic Markers in the Blood of Hepatitis B Virus Infected Patients

[0155] 1. Take 30 cases of hepatitis B patients (HBG), the diagnostic criteria are positive for HBV nucleic acid test or two or three items of HBV enzyme immunoassay, excluding any of liver cancer and other infections or diseases, fasting serum in the morning, within 1 hour Store at -80°C within one hour. Serum from 30 cases of healthy physical examination (Con) of the same age should be stored at -80°C within 1 hour. After the samples are collected, they will be uniformly processed according to the following methods.

[0156] 2. Serum sample processing method for reversed-phase chromatography analysis

[0157] 1) Plasma / serum samples stored at minus 80°C were thawed on ice at 4°C for 30-60 minutes.

[0158] 2) Take 100ul of serum to a labeled 1.5ml centrifuge tube, add 600ul of a 3:1 volume ratio of chloroform:methanol mixed solution, sonicate for 1 hour, add 100ul ...

Embodiment 2

[0190] The targeted detection of a group of metabolic markers in patients with hepatitis B virus mainly comprises the following steps:

[0191] 1. Take 70 cases of hepatitis B patients (HBV), the diagnostic criteria are positive for HBV nucleic acid test or two or three items of HBV enzyme immunoassay, excluding any of liver cancer and other infections or diseases, fasting serum in the morning, within 1 hour Store at -80°C, 30 cases of healthy physical examination serum (Con) of the same age, and store at -80°C within 1 hour. After the samples are collected, they are uniformly processed according to the following methods.

[0192] 2. Serum sample processing method for reversed-phase chromatography analysis

[0193] 1) Plasma / serum samples stored at -80°C were thawed on ice at 4°C for 30-60 minutes.

[0194] 2) Take 100ul of serum into a labeled 1.5ml centrifuge tube, add 10ul of internal standard, add 600ul of chloroform:methanol 3:1, sonicate for 1h, add 100ul of water, and ...

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Abstract

The invention belongs to the technical field of medicine biology, and relates to a screening method for a specific serum marker in the body of a patient, in particular to a screening method for a hepatitis B specific serum marker. The screening method comprises the following steps of collection and storage of serum samples, processing methods of the serum samples, positive phase and reversed phasechromatographic technical conditions, mass spectrum data acquisition and analysis, non-targeted metabolome data processing, result screening with significance difference, and verification and application of a screening result.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and relates to a screening method for heterosexual serum markers in patients, in particular to a screening method for hepatitis B specific serum markers. Background technique [0002] The cause of hepatitis B virus is hepatitis B virus, abbreviated as HBV, and hepatitis B virus is a DNA virus. Hepatitis B is an infectious disease mainly caused by hepatitis B virus. Clinically, the main manifestations are loss of appetite, nausea, upper abdominal discomfort, pain in the liver area, and fatigue. Some patients may have jaundice, fever and hepatomegaly accompanied by liver function damage. Some patients can become chronic and even develop into liver cirrhosis, and a few can develop into liver cancer. [0003] my country has a relatively high infection rate of hepatitis B virus. Before intervention measures such as hepatitis B vaccine vaccination and blood screening were implemented, t...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N33/576
CPCG01N30/88G01N33/5761G01N2030/8813
Inventor 张婷杨帆
Owner INST OF PATHOGEN BIOLOGY CHINESE ACADEMY OF MEDICAL SCI
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