Human sperm cryoprotectant containing D-trehalose and preparation method of human sperm cryoprotectant containing D-trehalose

A technology of cryoprotectant and trehalose, which is applied in the field of human sperm cryoprotectant and its preparation, can solve problems such as low recovery rate and decreased sperm motility, and achieve improved recovery rate, increased movement speed and motility index, and improved ability Effect

Inactive Publication Date: 2016-07-13
山西省人口计生委科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology improves how many times people have recovered their spins from an abortion process by increasing the percentage that they move faster or more easily during recovery compared with older methods without it. It also helps protect against damage caused by excessive exposure to oxidative stressors such as peroxynitrite (ONOO) gas produced when blood vessels are blocked due to certain medical conditions like cancer treatment). Overall, this new method provides better quality control for patients who recover them while still having healthy ones.

Problems solved by technology

Technological Problem addressed in this patents relates to reducing damaging effects caused during natural or artificial insemming procedures such as cryoconservations where high numbers of young sperms require quick treatment while maintaining their viability. Current solutions involve adding sugar molecules like sucrose to improve the efficiency of cryogenic treatments without causing harmful side effects. However, there has yet room for improvement due to its limited ability to effectively prevent ice growth from happening over time.

Method used

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  • Human sperm cryoprotectant containing D-trehalose and preparation method of human sperm cryoprotectant containing D-trehalose

Examples

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Effect test

Embodiment 1

[0032] A kind of human sperm cryoprotectant containing D-trehalose in the present embodiment contains the following components in the human sperm cryoprotectant per 100ml:

[0033] Glucose 1.5g, trisodium citrate dihydrate 1.3g, glycine 1.3g, glycerin 20ml, egg yolk 20ml, D-trehalose 2.0g and deionized water 60ml.

[0034] A method for preparing the human sperm cryoprotectant containing D-trehalose in the above-mentioned embodiment, it comprises the following steps:

[0035] 1) Add 1.5 g of glucose, 1.3 g of trisodium citrate dihydrate and 2.0 g of D-trehalose to 60 ml of sterile deionized water to prepare a mixed solution containing D-trehalose;

[0036] 2) Add 20ml of glycerin to the mixture, mix well, then add 1.3g of glycine, and stir well;

[0037] 3) After the substances in the above steps are completely dissolved, filter with a 0.22 μm fine-pore filter;

[0038] 4) Add 20ml of fresh egg yolk to the filtered solution;

[0039] 5) Incubate the solution with egg yolk in...

Embodiment 2

[0046] A kind of human sperm cryoprotectant containing D-trehalose in the present embodiment contains the following components in the human sperm cryoprotectant per 100ml:

[0047] Glucose 1.6g, trisodium citrate dihydrate 1.3g, glycine 1.3g, glycerin 20ml, egg yolk 20ml, D-trehalose 2.5g and deionized water 60ml.

[0048] A method for preparing the human sperm cryoprotectant containing D-trehalose in the above-mentioned embodiment, it comprises the following steps:

[0049] 1) Add 1.6 g of glucose, 1.3 g of trisodium citrate dihydrate and 2.5 g of D-trehalose to 60 ml of sterile deionized water to prepare a mixed solution containing D-trehalose;

[0050] 2) Add 20ml of glycerin to the mixture, mix well, then add 1.3g of glycine, and stir well;

[0051]3) After the substances in the above steps are completely dissolved, filter with a 0.22 μm fine-pore filter;

[0052] 4) Add 20ml of fresh egg yolk to the filtered solution;

[0053] 5) Incubate the solution with egg yolk in ...

Embodiment 3

[0060] A kind of human sperm cryoprotectant containing D-trehalose in the present embodiment contains the following components in the human sperm cryoprotectant per 100ml:

[0061] Glucose 1.55g, trisodium citrate dihydrate 1.3g, glycine 1.3g, glycerin 20ml, egg yolk 20ml, D-trehalose 2.2g and deionized water 60ml.

[0062] A method for preparing the human sperm cryoprotectant containing D-trehalose in the above-mentioned embodiment, it comprises the following steps:

[0063] 1) Add 1.55 g of glucose, 1.3 g of trisodium citrate dihydrate and 2.2 g of D-trehalose to 60 ml of sterile deionized water to prepare a mixed solution containing D-trehalose;

[0064] 2) Add 20ml of glycerin to the mixture, mix well, then add 1.3g of glycine, and stir well;

[0065] 3) After the substances in the above steps are completely dissolved, filter with a 0.22 μm fine-pore filter;

[0066] 4) Add 20ml of fresh egg yolk to the filtered solution;

[0067] 5) Incubate the solution with egg yolk ...

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Abstract

The invention relates to a human sperm cryoprotectant containing D-trehalose and a preparation method of the human sperm cryoprotectant containing the D-trehalose and mainly solves the technical problems of low thawing rate and sperm motility decline in use of existing human sperm slow cryoprotectants.The technical scheme includes that the every 100ml of the human sperm cryoprotectant comprises 1.5-1.6g of glucose, 1.3g of trisodium citrate dehydrate, 1.3g of glycine, 20ml of glycerin, 20ml of egg yolk, 2.0-2.5g of the D-trehalose and 60ml of deionized water.The preparation method includes: 1) adding the glucose, the trisodium citrate dehydrate and the D-trehalose to obtain a mixed solution; 2) adding the glycerin and the glycine; 3) using a filter for filtering; 4) adding the egg yolk; 5) incubating in a water bath; 6) detecting a pH value of the solution; 7) performing sterility test; 8) performing sperm survival test; 9) storing in a test tube at -70 DEG C.

Description

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Claims

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Application Information

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Owner 山西省人口计生委科学研究所
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