Drug-loaded ATP-sensitive liposome with tumor near-infrared fluorescence imaging function and preparation method thereof

Abstract

The invention relates to an ATP sensitive liposome with a tumor near infrared fluorescence development effect and a treatment effect. ATP sensitive nucleotide double strands loaded with adriamycin are encapsulated into a liposome dimolecular layer membrane coupled with tumor target polypeptide, so that a nanovesicle is formed, the ATP sensitive nucleotide double strands are formed by self-assembling of ATP sensitive nucleotide single strand coupled with fluorescent dye and ATP sensitive nucleotide complementary single strands coupled with a fluorescence quenching agent according to a base complementarity pairing principle, and the liposome dimolecular layer membrane coupled with the tumor target polypeptide consists of polyethylene glycol phosphatidyl ethanolamine coupled by the tumor target polypeptide, phosphatide and cholesterol. The invention further provides a preparation method of the liposome. The liposome concurrently has an active tumor targeting property, an ATP sensitive fluorescence emitting function, a medicine release switching function and an in vivo long-circulating function, the purpose of combining function like tumor near infrared fluorescence development, tumor diagnosis and tumor treatment in the same liposome is achieved, and besides, the preparation technology is simple and easy to operate.

Description

technical field [0001] The invention belongs to the field of tumor diagnosis and treatment, and in particular relates to a drug-loaded ATP-sensitive liposome with the function of tumor near-infrared fluorescence imaging and a preparation method thereof. Background technique [0002] Tumor has become a major disease that threatens human health. At present, when the tumor is diagnosed, the vast majority of cancer patients are in the middle and advanced stages, and the treatment methods mainly include surgery, radiotherapy, and chemotherapy. However, patients with advanced tumors are prone to recurrence, drug resistance, metastasis and complications after treatment, which are the main reasons for treatment failure and patient death. The "nano diagnosis and treatment system" developed in recent years integrates diagnosis and treatment on the same nanoprobe, and realizes high-sensitivity detection and minimally invasive / non-invasive treatment of tumors at the same time, with sim...

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Problems solved by technology

As a fluorescent probe, the micelles can be effectively delivered in vivo, enter cells, and have a high signal-low background ratio, excellent selectivity and biocompatibility, but there are the following problems: 1. Only rely on the enhanced penetration of nanometer size and The retention (EPR) effect reaches the tumor site, without active targeting, and the targeting efficiency is poor. The article does not provide in vivo targeting experiment data; 2. The PEG chain is too short to achieve the effect of prolonging the internal circulation; 3. It is necessary to synthesize oligo-containing Hybrid molecules of nucleotides, polyethylene glycol and di-fatty acyl lipids, with many synthetic steps, difficult synthesis and complex purification operations

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