Apparatus for multi-angle in situ spectrum detection of activity of peroxidase in tomato leaves, and method thereof

Abstract

The invention relates to the technical field of spectrum detection, and especially relates to a spectrum apparatus for detecting the activity and the content of peroxidase in tomato leaves, in the tomato planting field, and a method thereof. The apparatus comprises a spectrum probe, a light source, an omnibearing gyroscope and a light insulation device, and the omnibearing gyroscope comprises an arc suspension plate, an annular track plate, electric driving rollers and supporting legs. The method comprises the following steps: 1, preparing the apparatus; 2, carrying out spectrum blank correction; 3, carrying out automatic spectral angle adjustment sampling on the leaves; 4, carrying out multi-point sampling on the spectrum of the leaves; and 5, calculating physical and chemical values. The apparatus is arranged in the tomato planting field to obtain the spectrum data of the activity of the peroxidase in the tomato leaves of living plants; the apparatus can measure the tomato leaves in an omnibearing multi-angle manner; and the electric driving roller automatically rotates, and a camera cooperates to observe and monitor, so the detection efficiency is high.

Description

technical field [0001] The invention relates to the technical field of spectral detection, in particular to a spectral device for detecting peroxidase activity of tomato leaves at the tomato planting site. Background technique [0002] Tomato fruit contains a lot of nutrients such as carbohydrates, proteins, amino acids, vitamins and inorganic salts. Therefore, tomatoes are very popular among ordinary people in our country, and the annual output is very large. During the growth of tomato plants, the peroxidase activity in the leaves can resist the free radicals produced by abiotic stress, provide protection for plants, monitor the peroxidase activity indicators during the tomato growth process, and analyze the peroxidase activity indicators The reasons for the changes provide a basis for tomato planting decisions and a scientific basis for reasonable water and fertilizer management in tomato planting. [0003] The determination of tomato leaf peroxidase activity is still m...

AI Technical Summary

Problems solved by technology

The use of chemical determination methods is time-consuming and laborious, requires more labor costs, and the analysis process has more mechanical operations, and is generally not suitable for analyzing a large number of samples; chemical analysis requires the use of chemical reagents, which will inevitably pollute the environment. Due to the accuracy of data, convenient operation and environmental protection and pollution-free characteristics, the use of spectroscopy technology to replace conventional chemical analysis methods has been applied to a certain extent

[0004] like figure 1 As shown, due to the different growth shapes of tomato leaves, on-site measurement has high requirements on the projection angle of the light source and the incident angle of the spectral probe. Most of the existing spectral detection methods for tomato leaf peroxidase use picking new leaves, bagging crushed ice to keep fresh Prevent leaf deterioration to affect the accuracy of peroxidase spectrum measurement, which requires high real-time measurement of peroxidase activity

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