Device and method for detecting activity of superoxide dismutase of tomato leaves by in-situ spectrum

Abstract

The invention relates to the technical field of spectrum detection, and particularly relates to a spectrum device and a method for detecting the activity content of superoxide dismutase of tomato leaves in a tomato planting field. The device comprises a spectral probe, a light source, an omnibearing rotator and a light insulation device, wherein the omnibearing rotator comprises an arc suspending plate, an annular rail plate, an electric driving roller and supporting pins. The method comprises the following steps: (a) preparing equipment; (b) correcting a spectral whiteboard; (c) automatically adjusting and sampling the angle of leaf spectrum; (d) sampling multiple spots of the leaf spectrum; and (e) calculating the physical and chemical value. The device is arranged in the tomato planting field so as to acquire the spectral data of the activity of living superoxide dismutase of tomato leaves; the tomato leaves can be detected in an omnibearing manner; and the electric driving roller automatically rotates, and a camera is simultaneously matched for observing and monitoring, so that the detection efficiency is high.

Description

technical field [0001] The invention relates to the technical field of spectrum detection, in particular to a spectrum device for detecting the activity of superoxide dismutase in tomato leaves at the tomato planting site. Background technique [0002] Tomato fruit contains a lot of nutrients such as carbohydrates, proteins, amino acids, vitamins and inorganic salts. Therefore, tomatoes are very popular among ordinary people in our country, and the annual output is very large. During the growth of tomato plants, the activity of superoxide dismutase in leaves can resist free radicals produced by abiotic stress, provide protection for plants, monitor the indicators of superoxide dismutase activity during tomato growth, and analyze the causes of superoxide dismutase The reason for the change of activity index provides a basis for tomato planting decision-making, and provides a scientific basis for reasonable water and fertilizer management in tomato planting. [0003] The det...

AI Technical Summary

Problems solved by technology

The use of chemical determination methods is time-consuming and laborious, requires more labor costs, and the analysis process has more mechanical operations, and is generally not suitable for analyzing a large number of samples; chemical analysis requires the use of chemical reagents, which will inevitably pollute the environment. Due to the accuracy of data, convenient operation and environmental protection and pollution-free characteristics, the use of spectroscopy technology to replace conventional chemical analysis methods has been applied to a certain extent

[0004] like figure 1 As shown, due to the different growth forms of tomato leaves, on-site measurement has high requirements on the projection angle of the light source and the incident angle of the spectral probe. Most of the existing spectral detection methods for superoxide dismutase on tomato leaves use picking new leaves and bagging crushed ice. Keep fresh to prevent leaves from deteriorating to affect the accuracy of superoxide dismutase spectrum measurement, which requires high real-time measurement of superoxide dismutase activity

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