10 results about "Superoxide dismutase activity" patented technology

The present application provides a composition with satiety and weight control and anti-aging, and a preparation method and application thereof. The composition comprises the following raw materials by weight: polysaccharide 11-13 parts, fiber powder 20-25 parts, highland barley powder 12-18 parts, compound powder 18-23 parts, pectin 4-8 parts, ganoderma lucidum powder 6-9 parts, and compound protein 8-12 parts, and relates to the technical field of satiety and weight control and anti-aging compositions. The composition can form an effect of hindering nutrient absorption or prolonging gastric emptying, increase the volume of intestinal contents to achieve satiety, thereby achieving the functions of well controlling weight gain and reducing body weight. In addition, the composition can activate the body's antioxidant enzyme system, improve the activity of superoxide dismutase (SOD), and reduce the content of malondialdehyde (MDA), thereby delaying cell aging and achieving the anti-aging effect.

This invention provides a method and application for in-situ construction of barium titanate piezoelectric nanozymes based on mesoporous silica. Mesoporous silica (MSNs) is used as a carrier, titanium acetylacetonate as the titanium source, barium chloride as the barium source, and ferric chloride hexahydrate as the iron source. After stirring for 24 h and then gradient calcination for 6 h, iron-doped barium titanate piezoelectric nanozymes grown in situ within the pores of MSNs are synthesized. The prepared material exhibits piezoelectric catalytic activity, capable of converting endogenous H2O in tumor cells into hydroxyl radicals (•OH) and O2 into superoxide anions (•O2) under ultrasonic stimulation. ‑ It also possesses peroxidase-like (POD) and superoxide dismutase-like (SOD) activities, and can utilize SOD-like activity to convert •O2 ‑ The process involves converting the generated H2O2 into H2O2, alleviating the H2O2 deficiency within the tumor microenvironment. Then, utilizing POD-like activity, the generated H2O2 and endogenous tumor-derived H2O2 are converted into •OH, achieving a piezoelectric catalysis and enzyme cascade reaction to further increase the •OH level, efficiently killing tumor cells and inducing tumor cell death. This strategy allows for the in-situ development of iron-doped barium titanate piezoelectric nanozymes that simultaneously achieve sonodynamic catalytic therapeutic performance and enzyme catalytic therapeutic performance within the channels of MSNs.

The application discloses application of soybean peptides in fruit preservation. The application discloses application of soybean peptides in fruit preservation, expands the application range of the soybean peptides, and can significantly maintain the hardness, ethylene content, titratable acid content and soluble sugar content of apple fruits, maintain the superoxide dismutase activity of the fruits, reduce browning of the fruits, and enable long-term preservation of the apples and is safe and reliable.

This invention discloses a cryopreservation diluent containing fucoxanthin and its applications. Adding fucoxant to the cryopreservation diluent enhances the antioxidant capacity of frozen-thawed semen, reduces oxidative damage to goat sperm from reactive oxygen species during freezing-thawing, significantly improves sperm viability, motility, plasma membrane integrity, and total antioxidant capacity after freezing-thawing, enhances catalase and total superoxide dismutase activity, and reduces reactive oxygen species and malondialdehyde content. The effect is best when the fucoxant concentration is 2 μM during cryopreservation. The cryopreservation diluent of this invention is simple to prepare and operate, significantly improves the cryopreservation effect of semen, and has important practical application value.

This invention discloses a composite hydrogel with pH-responsive dual-enzyme activity switching properties, its preparation method, and its applications, belonging to the field of wound healing materials technology. The composite hydrogel comprises a nanozyme and a hydrogel. The nanozyme is a gold-platinum nanozyme, which is porous and dendritic, exhibiting different enzyme-like activities under different pH conditions. In an acidic environment, it exhibits oxidase-like and peroxidase-like activities, while in a neutral environment, it exhibits superoxide dismutase-like and catalase-like activities. This invention can exhibit reversibly switchable multi-enzyme mimicry activities under different pH conditions, thereby achieving dynamic synergistic regulation of antibacterial and antioxidant effects, overcoming the problem of difficulty in simultaneously achieving both in existing technologies. Furthermore, this invention has good biocompatibility, adhesion, and sustained-release properties, enabling adaptive functional regulation at different stages of wound healing, significantly promoting chronic wound healing and tissue repair, and showing promising clinical application prospects.

This invention belongs to the field of catalytic materials technology, specifically relating to a porous carbon biocatalytic material loaded with rhodium-iron bimetallic active sites, its preparation method, and its applications. The porous carbon biocatalytic material comprises a spiky porous carbon substrate, and rhodium clusters and iron single atoms uniformly loaded on its surface, forming Rh-Fe bimetallic active sites with charge transfer effects. The porous carbon biocatalytic material of this invention exhibits peroxidase-like and NADH oxidase-like activities under acidic conditions, and glucose oxidase-like, catalase-like, and superoxide dismutase-like activities under neutral conditions. It can efficiently kill bacteria and adsorb bacterial antigens, preventing macrophages from excessively polarizing to a pro-inflammatory state, ultimately promoting tissue regeneration and achieving ultra-rapid healing of inflammatory diabetic ulcers, with good biocompatibility.

This invention discloses an osmium nanozyme with highly efficient superoxide dismutase-like activity and its preparation method. The nanozyme is prepared by coordinating histidine with an osmium precursor followed by a reduction reaction, and features small particle size, good dispersibility, and structural stability. This nanozyme exhibits excellent SOD-like activity, with a specific activity reaching 5.04 × 10⁻⁶. 5 U / mg. The method described is mild, simple to operate, and has good reproducibility, and can be used for antioxidant-related research and applications.

This invention relates to the field of biomedical materials and catalytic materials, specifically to a hydrogen-bonded organic framework (HOF) with multi-enzyme activity and its preparation method. This material is prepared by co-assembling glucose oxidase (GOx), catalase (CAT), and manganese tetracarboxyphenylporphyrin (Mn-TCPP) and 1,4-phenyldicarboxamide hydrochloride, which have superoxide dismutase-like activity, to create a protein-hybridized HOF. This achieves the synergistic immobilization of natural enzymes and enzyme-like catalytic sites, thereby constructing an HOF catalytic system with multi-enzyme synergistic catalytic function. Under physiological conditions, this material can consume glucose, scavenge reactive oxygen species (ROS), and continuously release oxygen through multi-enzyme synergistic catalytic reactions, thereby regulating the pathological microenvironment and possessing application value in the repair of chronic wounds, especially in the treatment of diabetic chronic wounds.

This invention discloses a formulated feed and its application method for improving the antioxidant and high-temperature resistance of juvenile green abalone, belonging to the field of aquatic feed technology. The formulated feed includes a basic feed, γ-aminobutyric acid (GABA), and taurine. This invention prepares the formulated feed by designing the proportions of carbohydrates, proteins, and compound premixed feed in the basic feed, and by synergistically adding specific amounts of GABA and taurine. This formulated feed can effectively improve the activity of superoxide dismutase and glutathione peroxidase in juvenile green abalone under high-temperature conditions in summer, while enhancing their antioxidant and high-temperature resistance under high-temperature stress conditions. The survival rate of juvenile green abalone can reach up to 92.59%, which is significantly better than the traditional kelp feeding group. This provides a nutritional regulation scheme for solving the problem of large-scale mortality of juvenile green abalone during the high-temperature period in summer, and has good economic benefits and application prospects.

The application discloses a kind of noble metal coated silver-based core-shell structure nanomaterial, preparation method and application thereof.The method uses mild non-toxic aqueous system, utilizes green small molecule compound, silver source, tellurium or selenium source, gold or platinum source to realize one-pot preparation of noble metal coated silver-based core-shell structure nanocluster with enzyme-like activity and fluorescence effect.The reaction condition of the application is mild and controllable, simple to operate, and has good repeatability.The noble metal coated silver-based core-shell structure nanocluster prepared has good monodispersity, and has peroxidase-like, catalase-like, superoxide dismutase-like activity and fluorescence effect, can be adjusted to realize particle size, enzyme-like activity and fluorescence emission wavelength regulation, and is easy to surface functionalization modification, suitable for a variety of biomedical detection scenarios.