89 results about "Superoxide dismutase activity" patented technology

The invention discloses a continuous cropping Chinese cabbage planting method based on a microbial agent and application, and belongs to the technical field of crop cultivation. According to the planting method, Chinese cabbage and garlic are intercropped according to the ratio of 1: 1, and a trichoderma harzianum agent is applied. Results show that the planting method can significantly increase the growth indexes such as the plant height, the root length, the leaf chlorophyll content and the soluble sugar content of the Chinese cabbage; the soluble protein content, the peroxidase activity, the superoxide dismutase activity and the malondialdehyde content of the Chinese cabbage leaves are obviously reduced. Meanwhile, the content of rapidly available phosphorus, rapidly available potassium, effective calcium and other substances in the soil can be increased; the activities of urease, catalase and alkaline phosphatase in the soil are also obviously improved. In addition, the number of fungi, bacteria and actinomycetes in the soil is also remarkably increased. Therefore, according to the planting method, the quality of continuous cropping soil can be improved, Chinese cabbage growth is promoted, and theoretical support is provided for healthy and sustainable development of the Chinese cabbage industry.

The invention discloses a method for evaluating and screening salt tolerance of triticale germplasm resources in a germination stage, and belongs to the technical field of evaluation and screening of plant salt tolerance, salt stress concentration screening: triticale seeds are treated by NaCl solutions with different concentrations, indexes such as survival rate, plant height and overground part fresh weight are measured, the NaCl solution with the concentration of 200 mmol. L <-1 > is determined as the salt tolerance evaluation appropriate concentration, and the salt stress concentration is determined as the salt tolerance evaluation appropriate concentration. Salt tolerance evaluation test: carrying out salt stress treatment by adopting a 200mmol. L <-1 > NaCl solution, determining phenotypic indexes such as survival rate, plant height, fresh weight of overground part, root length and chlorophyll content, and physiological and biochemical indexes such as catalase, peroxidase and superoxide dismutase activity and malondialdehyde content, and carrying out principal component analysis, membership function analysis and clustering analysis to obtain a salt tolerance evaluation result. Calculating a salt tolerance comprehensive evaluation value, and screening salt tolerance triticale germplasm resources.

The invention discloses marasmius albus DCY6478 as well as a culture medium and application thereof, and belongs to the technical field of microorganisms. The invention discloses a Marasmilla sp. DCY6478 strain, and the preservation number of the Marasmilla sp. DCY6478 strain is GDMCC (China General Microbiological Culture Collection Center) No.65883. The Marasmilla sp. Experimental verification results show that the culture medium formula most suitable for growth of the white marasmius is prepared from the following components in percentage by weight: 40% of apple sawdust, 36% of corncob, 14% of cottonseed hull, 1% of lime and 1% of gypsum; the fermentation liquor is verified to have an inhibition effect on a plurality of pathogenic bacteria; the DPPH free radical scavenging capacity of the fermented hyphae is verified, the superoxide dismutase activity and catalase activity are both higher than those of sporocarp, and the fermented hyphae can be used as a natural antioxidant source; it is verified that fermented hyphae or sporocarp of the strain have the capacity of secreting amino acid and polysaccharide and producing crude protein, and a new raw material selection is provided for the industries of food, medicine and the like.

The present invention discloses a preparation method of CFS nanozyme. This nanozyme has broad-spectrum bactericidal property, has low toxicity to normal cells, and there is no problem of bacterial drug resistance, and is expected to be used in the field of antibiotic-free sterilization. The CFS-ion-MN wound repair microneedle patch provided by the present invention, as a wound dressing, can transdermally deliver the antibacterial CFS nanozyme to the deep part of the wound, effectively kill bacteria. The CFS nanozyme can also inhibit inflammation in the body through its superoxide dismutase-like activity, promote wound healing, and promote the antibacterial and healing of the wounds of mice infected with Staphylococcus aureus. Among them, Co 2+ and Fe 3+ can inhibit the IL-17 and NF-κB signaling pathways, thereby accelerating wound closure.

The invention discloses application of polydatin in preparation of a medicine for relieving hepatotoxicity caused by zearalenone, and belongs to the technical field of biological medicine. The invention reveals that polydatin inhibits hepatotoxicity caused by zearalenone through targeted xanthine oxidase for the first time, so that the toxicity of zearalenone to an organism is relieved. Polydatin can be directly combined with xanthine oxidase to inhibit the catalytic activity of xanthine oxidase, so that the oxidative stress injury of the liver after ZEA exposure is reduced, meanwhile, the content of malondialdehyde (MDA) in the liver can be remarkably reduced, the activity of superoxide dismutase (SOD) is improved, the liver oxidation-antioxidant balance is recovered, and liver cells are protected from oxidative injury of ZEA. The invention provides a new scientific basis and development direction for application of polydatin in the fields of mycotoxin detoxification, oxidation resistance, liver protection and the like.

Complexes of polyoxometalate (POM) cluster anions and metal oxide nanocrystal (NC) cores (POM-NC) with tunable NC surface protonation are disclosed. These protonated POM-NC complexes enable precise control over catalytic properties, enhancing catalytic activity under near-neutral pH conditions while improving stability and solubility across various applications. The protonated POM- metal oxide complexes facilitate the activation of airborne O2 under ambient conditions and the production of reactive oxygen species (ROS), including hydroxyl radicals, superoxide, and peroxides. In certain catalytic processes, these complexes mimic enzymatic functions, such as oxidase, peroxidase, catalase, and superoxide dismutase activity, making them valuable for various biological, therapeutic, chemical and environmental applications such as applications related to oxidative catalysis and ROS scavenging.

The invention is suitable for the technical field of pesticide residue detection, and provides a method for distinguishing and detecting pesticide based on copper-platinum bimetallic nano-enzyme. According to the method, a colorimetric sensing array with three sensing channels is constructed by synthesizing a copper-platinum bimetallic nano-enzyme (CuPtNPs) with oxidase-like activity, laccase-like activity and superoxide dismutase-like activity. A fingerprint spectrum is obtained based on specific effects of different pesticides and enzyme-like activities, and qualitative distinguishing and quantitative detection of various pesticides are realized by combining a machine algorithm. According to the method, nine pesticide residues can be measured at a time, six of the nine pesticide residues can be quantitatively analyzed, and the problem that a traditional method only can be used for single detection is solved. A strategy of constructing three channels based on a single nano-enzyme receptor CuPtNPs has the remarkable advantages of simple synthesis process, convenient operation flow and low detection cost, is good in applicability in actual agricultural product samples such as Chinese cabbages and apples, and provides an efficient solution for high-throughput detection of pesticide residues.

The invention discloses preparation of an antibacterial copper-based nano-enzyme fiber dressing capable of promoting wound healing, and belongs to the technical field of medical use. According to the method, 5, 6-dimethylbenzimidazole and copper salt are used as raw materials, the copper-based nano-enzyme is synthesized through a hot bath reaction method of a solvent, reactants are uniformly dispersed in a solution, and the defects that traditional nano-enzyme element components are not uniform, and active sites are low in density are overcome. The prepared copper-based nano enzyme has a unique metal active center, and has oxidase-like activity, catalase-like activity and superoxide dismutase-like activity. According to the copper-based nano-enzyme Cu-NPs II, the activity of oxidase can be achieved without hydrogen peroxide, active oxygen can be generated to achieve the bactericidal effect, when excessive ROS is generated, superoxide dismutase-like activity of the copper-based nano-enzyme Cu-NPs II converts the excessive ROS into H2O2, and due to the fact that the copper-based nano-enzyme Cu-NPs II has catalase-like activity and can decompose H2O2 into water and oxygen, the copper-based nano-enzyme Cu-NPs II has good microenvironment adjusting capacity.

The invention relates to the technical field of nano-materials, and discloses a heterogeneous diatomic nano-enzyme as well as a preparation method and application thereof. The invention provides a space confinement regulation and control strategy which is used for constructing a heterodiatomic catalytic center on a nitrogen-doped carbon skeleton and preparing nano-enzyme with structural stability and synergistic catalytic performance. According to the method, a confinement framework formed by self-assembly of first metal ions such as cobalt ions and second metal ions such as zinc ions and organic ligands is used as a template, third metal ions such as iron ions are introduced, and then carbonization heat treatment is performed in a protective gas atmosphere, so that bimetal sites with stable bridging configuration are formed in situ. The obtained nano-enzyme has the catalytic activity of various antioxidant enzymes, including catalase and remarkable superoxide dismutase activity, and has more excellent free radical scavenging capacity compared with a single-metal or non-bridging system.

The invention belongs to the technical field of microbial agents, and particularly relates to a composite microbial agent, a composite liquid microbial agent and application. The invention provides a complex microbial inoculant, which is prepared from bacillus mojavensis W1, bacillus halotolerans L21 and bacillus subtilis M4, wherein the complex microbial inoculant is prepared from bacillus mojavensis, bacillus halotolerans L21 and bacillus subtilis M4, and the complex microbial inoculant is prepared from bacillus mojavensis, bacillus halotolerans L21 and bacillus subtilis M4. The bacillus mojavensis W1, the bacillus halotolerant L21 and the bacillus subtilis M4 in the complex microbial inoculant interact with one another, so that the defense mechanism of plants can be activated, the activity of superoxide dismutase (SOD), the activity of peroxidase (POD) and the activity of catalase (CAT) can be improved, and the content of malondialdehyde (MDA) can be reduced, thereby improving the prevention and treatment effect on the root rot of the cornutgionium axentgii.

Provided is a test method with which mild cognitive impairment (MCI) is detectable and a measurement reagent for use in the test method. A test method for MCI according to the present invention includes a step of measuring the activity of superoxide dismutase (SOD) in a biological sample of a subject.

The application provides application of ABT199 in preparation of a drug for treating muscle atrophy. Compared with the prior art, the application provides a new use of ABT199 in treating muscle atrophy. Experimental research proves that ABT199 administration can significantly restore the exercise capacity of a cachexia-induced muscle atrophy mouse, improve the muscle cross-sectional area of the mouse, restore the reduced glutathione content of the mouse, and improve the superoxide dismutase activity, so as to improve the muscle atrophy phenotype of the mouse and muscle atrophy-related biochemical indexes. ABT199 can significantly restore cell muscle atrophy-related gene and protein expression, and improve the cachexia-induced muscle atrophy phenotype by reducing related inflammatory mediators; ABT199 can significantly improve the mitochondrial membrane potential, increase the expression of mitochondrial neogenesis-related genes, and reduce the production of active oxygen.

The invention belongs to the technical field of microbial control, and particularly relates to a salt-tolerant bacillus, a microbial agent, a preparation method, a control method and application. The invention provides a salt-tolerant bacillus L21 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.35478. The salt-tolerant bacillus L21 can activate the defense mechanism of plants, improve the activity of defensive enzymes in the plants, and enable the plants to have higher oxidation resistance when encountering pathogenic bacteria, so that ROS accumulation is more effectively reduced, MDA is reduced, and the morbidity of root rot is reduced. The result of the embodiment shows that the bacillus halotolerant L21 can improve the activity of superoxide dismutase (SOD), the activity of peroxidase (POD) and the activity of catalase (CAT), reduce the content of malondialdehyde (MDA) and reduce the morbidity of root rot of cornutgionium axataenense.

The invention discloses application of ellagic acid in preparation of a medicine for preventing or treating ionizing radiation injury, and belongs to the technical field of biological medicine and radiation protection. Experiments prove that ellagic acid with an effective concentration can inhibit lipid peroxidation, enhance the activity of glutathione transferase and superoxide dismutase and promote the expression of antioxidant related genes such as sod-1, sod-2, gst-1 and gst-29 and immune related genes such as lys-1, irg-1, irg-2 and mul-1, so that the ellagic acid with an effective concentration can inhibit lipid peroxidation and improve the activity of glutathione transferase and superoxide dismutase. Therefore, oxidative damage, inflammatory response, reduced stress tolerance, accelerated aging and shortened service life caused by ionizing radiation exposure are relieved, and the compound can be applied to preparation of drugs for preventing or treating ionizing radiation damage and is suitable for nuclear accident emergency protection, space radiation protection of astronauts and adjuvant therapy of tumor radiotherapy patients. The invention provides a theoretical basis for developing an efficient and low-toxicity ionizing radiation protective agent based on ellagic acid, and has a wide application prospect.

The invention belongs to the technical field of microbial prevention and control, and particularly relates to bacillus subtilis, a microbial agent, a preparation method, a prevention and control method and application. The invention provides a strain of bacillus subtilis M4, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.35477. The bacillus subtilis M4 is used for preventing and treating root rot of cornutgionium axe. The bacillus subtilis M4 can improve the activity of disease-resistant defensin superoxide dismutase (SOD), the activity of peroxidase (POD) and the activity of catalase (CAT), reduce the content of malondialdehyde (MDA), finally reduce the morbidity and the morbidity index of plant root rot and promote plant growth.

The invention belongs to the technical field of protein engineering, particularly relates to a protein tag with superoxide dismutase activity and application, and provides a protein tag which comprises polypeptide or protein with superoxide dismutase activity. On the second aspect, a fusion protein is provided and comprises the protein tag described in the first aspect and at least one target polypeptide or protein, the target polypeptide or protein is not superoxide dismutase, and the target polypeptide or protein is not superoxide dismutase only by using the protein tag disclosed by the invention. According to the present invention, the technical effects of target polypeptide or protein expression quantity detection (SOD enzyme activity detection), target polypeptide or protein protection from oxidative damage, target polypeptide or protein stability improvement and target polypeptide or protein solubility improvement can be simultaneously achieved without adding any additional step compared with the fusion protein expression operation in the prior art; the operation is convenient.

The invention discloses calf animal coccus PD13 for improving Alzheimer's disease and application of the calf animal coccus PD13, and relates to the technical field of biology. Experiments prove that in an Alzheimer's disease model mouse induced by injecting A beta oligomer into the brain, the Azococcus calvus PD13 can improve spatial learning and memory disorder and autonomous exploration ability of the AD model mouse and relieve damage to neurons in a hippocampal region of the Alzheimer's disease model mouse, so that the effect of protecting the neurons is achieved, and the Azococcus calvus PD13 can be used for preparing a medicine for treating the Alzheimer's disease. According to the present invention, with the application of the animal coccus calves, the content of glutathione in mouse serum and the enzyme activity of superoxide dismutase are increased, and the content of malondialdehyde is reduced, such that the animal coccus calves provides good prevention and treatment effects on the Alzheimer's disease, and the new approach is provided for developing the Alzheimer's disease-related drugs.

The invention discloses indigowoad root derived carbon dots as well as a preparation method and application thereof, and belongs to the technical field of novel biomedical materials. The method comprises the following steps: generating a carbonized product by a one-step hydrothermal method, and separating and purifying to obtain the indigowoad root-derived carbon dots. The carbon dots do not contain metal elements, are non-toxic and high in biological safety, and show good free radical scavenging activity, certain superoxide dismutase-like activity and good biocompatibility no matter in vitro or in vivo. On the basis, the indigowoad root-derived carbon dots also show excellent anti-inflammatory activity, can down-regulate the inflammatory level of cells, relieve the paralysis symptom of AD nematode, relieve LPS-induced memory deterioration of mice and treat LPS-induced mouse Alzheimer's disease, and can be further applied to preparation of drugs for treating neurodegenerative diseases.

The present application provides a composition with satiety and weight control and anti-aging, and a preparation method and application thereof. The composition comprises the following raw materials by weight: polysaccharide 11-13 parts, fiber powder 20-25 parts, highland barley powder 12-18 parts, compound powder 18-23 parts, pectin 4-8 parts, ganoderma lucidum powder 6-9 parts, and compound protein 8-12 parts, and relates to the technical field of satiety and weight control and anti-aging compositions. The composition can form an effect of hindering nutrient absorption or prolonging gastric emptying, increase the volume of intestinal contents to achieve satiety, thereby achieving the functions of well controlling weight gain and reducing body weight. In addition, the composition can activate the body's antioxidant enzyme system, improve the activity of superoxide dismutase (SOD), and reduce the content of malondialdehyde (MDA), thereby delaying cell aging and achieving the anti-aging effect.

The invention belongs to the field of medicines, and relates to a scutellarin aglycone derivative as well as a preparation method and application thereof. The scutellarin aglycone derivative has a structure as shown in a formula I. The preparation method comprises the following steps: reacting scutellarin (a compound II) with methanol to obtain a compound III, then reacting with bornyl chloroacetate (a compound IV) to obtain a compound V, and finally carrying out an alkaline hydrolysis reaction to obtain the compound I, the scutellarin aglycone derivative has the effect of preventing or treating cardiovascular and cerebrovascular related diseases, can improve the activity of superoxide dismutase (SOD), inhibit the production of lactic dehydrogenase (LDH) and reduce the malondialdehyde (MDA) level superior to that of scutellarin, has the inhibiting effect on inflammatory factors TNF-alpha, IL-6 and IL-1beta in cell supernatant superior to that of scutellarin, and can be used for preparing a medicine for treating cardiovascular and cerebrovascular related diseases, such as cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases, cardiovascular and cerebrovascular related diseases and cardiovascular and cerebrovascular related diseases. The invention has the potential of preparing or preventing cardiovascular and cerebrovascular related diseases.

The invention discloses application of metal phthalocyanine artificial antioxidant enzyme in preparation of drugs for preventing and treating oxidative stress diseases. The metal phthalocyanine derivative is used as the artificial antioxidant enzyme, superoxide free radicals (O2 <.->) are efficiently removed, reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) are removed in the presence of glutathione, and the metal phthalocyanine derivative shows superoxide dismutase (SOD)-like activity and glutathione peroxidase (GPx)-like activity. The artificial antioxidant enzyme has good biocompatibility, can effectively regulate and control the oxidative stress level of an inflammation part, reduces the expression level of inflammatory cytokines, and remarkably improves the symptoms of oxidative stress diseases of an animal model. The artificial antioxidant enzyme can improve treatment of ROS related diseases such as inflammatory bowel diseases, and a new idea is provided for application of the antioxidant enzyme.

The invention discloses a hydrogel microneedle loaded with a photo-thermal Fe3O4-MXene nano material as well as preparation and application of the hydrogel microneedle, and belongs to the field of biological medicines. The invention finds that a Fe3O4-Mxene nano material mainly takes CAT enzyme under a weak alkaline condition and can quickly remove hydrogen peroxide, Fe3O4 and TI3C2 form a unique heterojunction structure, the photo-thermal conversion performance of Ti3C2, the catalase activity of Fe3O4 and the superoxide dismutase activity of Fe3O4 are improved, and removal of Fe3O4 to active oxygen is promoted. The hydrogel microneedle loaded with the photo-thermal Fe3O4-MXene nano material, provided by the invention, is applied to diabetic, infectious and chronic wound surfaces, can remove excessive active oxygen, improves local oxygen deficit and promotes proliferation and regeneration of skin, and has a good application effect.

The invention discloses a copper ion / tyrosine nano-enzyme with both superoxide dismutase activity and catalase activity and a preparation method thereof. The nano-enzyme is formed by coordination self-assembly of copper ions and tyrosine according to a molar ratio of 1: 2, and has dual catalytic activities of superoxide dismutase and catalase. The preparation method comprises the following steps: dissolving tyrosine in deionized water, adding copper acetate, reacting in a water bath at 60-80 DEG C for 12-24 hours, and centrifuging to obtain the nano-enzyme. The nano-enzyme shows excellent oxidation resistance, has good biocompatibility and catalytic stability, and can be widely applied to the fields of active oxygen removal, oxidative stress intervention, biomedical material and antioxidant drug development and the like.

The invention discloses a method for evaluating the toxicity of a lithium ion battery cathode material lithium cobalt oxide to plants. The method comprises the following steps: preparing LCO with a nanosheet layer structure; simulating a plant root microenvironment, and analyzing physicochemical characteristics of LCO in the plant root microenvironment; the LCO is exposed in a target plant growth system, and the environmental toxicity of the LCO to the plant is comprehensively judged by detecting phenotypic indexes, photosynthesis parameters, root ultrastructure integrity, active oxygen distribution, malondialdehyde content and superoxide dismutase activity of the plant. The method for evaluating the toxicity of the lithium cobalt oxide serving as the positive electrode material of the lithium ion battery to the plants has important significance on revealing the ecological risk of the positive electrode material of the battery and establishing a standardized detection technology system.

The invention discloses a preparation method and application of a polygonatum sibiricum leaf extract antioxidant composition, and belongs to the technical field of natural product extraction and utilization. The method comprises the following steps: by taking waste polygonatum kingianum leaves as a raw material, cleaning, drying at low temperature, breaking walls and crushing, heating, refluxing and extracting by adopting pure water, and performing low-temperature high-speed centrifugal purification and vacuum freeze drying to obtain the high-activity composition. According to the core technology, various active substances such as total sugar, flavone and phenylpropanoid in the polygonatum kingianum leaves are enriched and reserved to the maximum extent through a full-water-based and solvent-free green process, and a synergistic anti-oxidation system is formed. The composition can effectively remove DPPH and ABTS free radicals, inhibit generation of active oxygen in keratinocytes induced by UVB radiation and improve the activity of superoxide dismutase in the cells, and has definite in-vitro anti-oxidation and cell light protection effects. According to the invention, agricultural wastes are recycled, the process is safe, the cost is low, and the obtained composition can be applied to skin antioxidant products such as face cream, essence and the like.

The invention relates to a system for improving the activity of superoxide dismutase based on natural product extracts and a preparation method and application thereof.A quaternary synergistic system with cabbage powder, roxburgh rose powder, pork liver powder and psyllium husk powder as cores is provided for the first time, roxburgh roses provide high-activity SOD, cabbages activate an Nrf2 pathway to promote synthesis of endogenous SOD, and the activity of superoxide dismutase is improved; according to the present invention, the two channels such as exogenous supplement and endogenous activation are achieved, and under the action of the two channels such as exogenous supplement and endogenous activation, the content of superoxide dismutase (SOD) is increased, such that the related components can be effectively retained, and the activity of the superoxide dismutase (SOD) can be improved; pork liver powder supplements Cu / Zn elements, dietary fibers of semen plantaginis husk powder enhance absorption, and synergy of cofactors and carriers is achieved; the problems that existing enzymes are prone to inactivation (gastric acid degradation), large in molecular weight (low in absorption rate), high in cost, limited in action target spot of a single component and the like are solved.

The invention discloses a pharmaceutical composition for preventing and / or treating acute pancreatitis. The Anarinone root plays a role in preventing and treating acute pancreatitis by inhibiting oxidative stress (such as improving the activity of superoxide dismutase (SOD) and reducing the content of malondialdehyde (MDA)) and / or inhibiting inflammatory response (such as inhibiting activation of an NF-kappa B signal channel and reducing expression of proinflammatory factors such as IL-6, TNF-alpha and the like); compared with a chemical synthetic medicine octreotide (possibly causing gastrointestinal reaction, abnormal blood glucose and other side effects), the long-term use experience of the Anacarid root serving as a traditional habitual natural medicinal material shows that the Anacarid root possibly has better biocompatibility and lower adverse reaction risk, and a possibly safer treatment choice is provided for patients.

The invention relates to the technical field of crop planting, and provides SOD (superoxide dismutase) and organic selenium double-enhanced functional rice and a probiotic ecological cultivation method thereof. Comprising the following steps: preparing an SOD (superoxide dismutase) solution, preparing a compound probiotic fermentation solution, preparing an organic selenium mixed solution, soaking seeds, growing seedlings in a greenhouse, managing in a seedling stage of the greenhouse, conditioning before field transplanting, treating seedlings in a transplanting stage, treating in a growing stage of the field, and harvesting rice. The synergistic enrichment of SOD and organic selenium in rice is realized through the absorption promotion of probiotics, the directional induction of an SOD superoxide dismutase solution, the precise application of an organic selenium mixed solution, the whole ecological planting process and the reduction of environmental pollution, and the two indexes are both superior to those of the existing single strengthening technology. The rice still contains SOD (superoxide dismutase) activity, and meets the requirements of green agriculture and sustainable development.

The invention discloses a method for detecting kiwi fruits infected with botryosphaeria dothidea based on antioxidant substances. The ratio of the superoxide dismutase activity to the flavonoid content in the kiwi fruits is used for distinguishing the fruits infected by botryosphaeria dothidea and the fruits not infected by the botryosphaeria dothidea in the storage process, and the fruits with the ratio of the superoxide dismutase activity to the flavonoid content in the to-be-detected sample being 310 or above are diseased fruits infected by the botryosphaeria dothidea; the fruits with the ratio of the superoxide dismutase activity to the flavonoid content being lower than 250 are healthy fruits. The method is simple to operate and reliable in detection result, and can provide reference for judging whether the kiwi fruits are infected by pathogenic bacteria or not in the storage process.

The invention belongs to the technical field of aquatic immunity and biological preparations, and discloses a CXCL20a protein mutant and application of the CXCL20a protein mutant as a grass carp reovirus inactivated vaccine adjuvant. In order to improve the immunomodulatory activity of CXCL20a, the applicant constructs and obtains a CXCL20a mutant protein. After the mutant and a GCRV-II type inactivated vaccine are combined to immunize grass carp, the expression of immune related genes can be remarkably promoted, the activity of serum acid phosphatase and superoxide dismutase is enhanced, and meanwhile, the level of lactic dehydrogenase is reduced. A challenge experiment proves that the survival rate of the grass carp combined with the inactivated vaccine for immunization is increased by about 8% compared with that of a non-mutant combined group, and the in-vivo virus load is reduced. As an immunologic adjuvant, the CXCL20a mutant M-C20 provided by the invention can effectively enhance early immune response and overall protection effect in vaccine immunity, and has good safety, stability and application prospect.