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Clavibacter michiganensis multiplex detection reagent and kit, and application thereof

A multiple detection, coryneform bacteria technology, applied in the direction of microbial determination/inspection, microorganisms, biochemical equipment and methods, etc., can solve problems such as easy to cause confusion, to avoid the spread of Corynebacterium Michigan, improve detection efficiency, improve The effect of detection quality

Active Publication Date: 2016-08-24
SHENZHEN AUDAQUE DATA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Because the morphological and molecular characteristics of the above five Corynebacterium michigani are very similar, it is easy to cause confusion. Therefore, there is an urgent need for a method or reagent that can effectively detect and identify Corynebacterium michigani

Method used

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  • Clavibacter michiganensis multiplex detection reagent and kit, and application thereof
  • Clavibacter michiganensis multiplex detection reagent and kit, and application thereof
  • Clavibacter michiganensis multiplex detection reagent and kit, and application thereof

Examples

Experimental program
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Effect test

Embodiment

[0046] The Corynebacterium michiganensis multiple detection reagent in this example consists of five subspecies of Corynebacterium michiganensis: Clavibacter michiganensis subsp.nebraskensis, Clavibacter michiganensis subsp.michiganensis, Clavibacter michiganensis subsp.insidiosus, Clavibacter michiganensis subsp.sepedonicus, Clavibacter michiganensis subsp. Tessellarius' five lock probes. In this example, rolling circle amplification combined with a liquid phase chip was used to conduct specificity tests on five padlock probes. The design and related tests of the five padlock probes are detailed as follows:

[0047] 1. Test materials and main solutions

[0048] 1. Tested strains

[0049] In this example, two strains of CMI, three strains of CMM, three strains of CMN, two strains of CMS and two strains of CMT were used for the test. At the same time, seven strains of other similar species were used as negative controls for the test. The details of the strains are shown in Ta...

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Abstract

The invention discloses a Clavibacter michiganensis multiplex detection reagent and kit, and application thereof. The Clavibacter michiganensis multiplex detection reagent contains five padlock probes which are respectively a CMN padlock probe disclosed as SEQ ID No.1, a CMM padlock probe disclosed as SEQ ID No.2, a CMI padlock probe disclosed as SEQ ID No.3, a CMS padlock probe disclosed as SEQ ID No.4 and a CMT padlock probe disclosed as SEQ ID No.5. The multiplex detection reagent contains five subspecies of specific padlock probes capable of simultaneously carrying out specific detection on five subspecies. The reagent enhances the detection efficiency and detection quality. The reagent and kit provide a simple effective way for Clavibacter michiganensis high-flux detection, is suitable for inspection and quarantine departments, and provides powerful scientific references for ensuring biosafety and avoiding pathogene transmission.

Description

technical field [0001] The application relates to the field of detection of plant pathogenic bacteria, in particular to a multiple detection reagent, kit and application of Corynebacterium michigani. Background technique [0002] Clavibacter michiganensis belongs to Actinomycetes, Micrococcaceae, and the genus Clavibacter michiganensis, and is an important plant pathogenic bacterium. Among them, five subspecies of Corynebacterium Michigan: Clavibacter michiganensis subsp.nebraskensis (abbreviated CMN), Clavibacter michiganensis subsp.michiganensis (abbreviated CMM), Clavibacter michiganensis subsp.insidiosus (abbreviated CMI), Clavibacter michiganensis subsp.sepedonicus (abbreviated CMS ), Clavibacter michiganensis subsp. tessellarius (abbreviated as CMT), are the focus of current inspection and quarantine research on Corynebacterium michiganensis. These five subspecies belong to the harmful and widely distributed Corynebacterium michigani subspecies. Moreover, the five sub...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12R1/15
CPCC12Q1/689C12Q2600/16C12Q2600/166
Inventor 冯建军王忠文吴绍精龙海陈枝楠
Owner SHENZHEN AUDAQUE DATA TECH
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