Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Salvia miltiorrhiza and safflower composition and its preparation method and application

A composition, the technology of safflower, a new application in the preparation of antithrombotic disease drugs, the composition of Danshen and safflower and its extraction field, can solve the shortage of standard products, which cannot be widely and accurately used to identify traditional Chinese medicine complex components, expensive detection costs, etc.

Active Publication Date: 2020-05-08
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, LC-MS has identified more chemical components through analysis strategies such as the difference in collision energy, characteristic ion filtering, and energy neutral loss. However, these methods are not yet extensive and accurate due to the serious shortage of standards and the high cost of detection. for the identification of complex components of traditional Chinese medicine

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Salvia miltiorrhiza and safflower composition and its preparation method and application
  • Salvia miltiorrhiza and safflower composition and its preparation method and application
  • Salvia miltiorrhiza and safflower composition and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Instruments and reagents

[0023] American Waters 2695 high performance liquid chromatography system, Waters 2998PDA detector, Empower data management system, 16 desktop centrifuges (Beckman Coulter, USA), FW80 high-speed universal centrifuge (Tianjin Test Instrument Co., Ltd.), YRE-301 rotary evaporator (Gongyi Yuhua Instrument Co., Ltd.), BT125 electronic balance (Germany Sartorius Scientific Instrument Co., Ltd.), EPED ultrapure water system (Nanjing Epod Technology Development Co., Ltd.).

[0024] Acetonitrile (Scharlab, Spain) was a chromatographically pure reagent, methanol (Nanjing Chemical Reagent Co., Ltd.), and formic acid (Merck, Germany) were analytically pure, and ultrapure water (self-made). Reference substances: salvianolic acid B (DFB, batch number 111562) and tanshinone IIA (DST, batch number 110766) were purchased from China Institute for the Control of Pharmaceutical and Biological Products; hydroxysafflower yellow A (HSYA, batch number 140827) w...

Embodiment 2

[0063] Embodiment 2 antithrombotic contrast experiment

[0064] Effects of Salvia Miltiorrhiza and Safflower in Different Weight Ratio on Coagulation Time

[0065] At present, the conventional method for screening antithrombotic compounds is to investigate the activity of the compound on inhibiting platelet aggregation and its influence on the coagulation time. The present invention examines the antithrombotic activity of each compound by measuring the influence of the compound on the coagulation time.

[0066] Specific method: take healthy male rabbits, inject 30mg / kg rabbit body weight pentobarbital sodium saline solution into the ear vein for anesthesia, separate the common carotid artery by operation, collect blood, collect it in a plastic centrifuge tube, add 3.8% citric acid Sodium aqueous solution anticoagulant (volume ratio of blood to anticoagulant is 9:1). Centrifuge at 800r / min for 10min to prepare platelet-rich plasma (Platelet-rich plasma, PRP), and centrifuge at...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a composition with salviae miltiorrhiza and safflower. The weight ratio of salviae miltiorrhiza to safflower is (5 to 1) to (5 to 2). Through intensive research on a large number of ancient and modern prescriptions and traditional Chinese medicine application data, active components in salviae miltiorrhiza and safflower are classified according to chemical structure types on the basis of salviae miltiorrhiza and safflower to the existing research; the HPLC-PDA technique is adopted; a chemical substance library is built; a chemical fuzzy recognition strategy is introduced; separated chromatographic peaks of a chromatogram are used for classifying and identifying components and structures according to a characteristic ultraviolet absorption spectrum; dynamic variation rules of different types of chemical components under different preparation methods and different weight ratios are researched by designing symmetry ratios; a weight ratio medicine pair of salviae miltiorrhiza and safflower with optimal weight ratio is screened; the experiment result shows that the preferably-prepared composition with salviae miltiorrhiza and safflower has a good function of resisting cardiovascular and cerebrovascular diseases.

Description

technical field [0001] The invention relates to a traditional Chinese medicine composition, in particular to the composition of salvia miltiorrhiza and safflower, its extraction method and its new application in the preparation of antithrombotic disease medicine. Background technique [0002] "Drug pair" is a commonly used form of compatibility in clinical prescriptions of traditional Chinese medicine, and it is the sublimation of the clinical medication experience accumulated by physicians of all ages. The medicine pair is a bridge connecting a single traditional Chinese medicine and several prescriptions. It is the essence and core of prescription compatibility. It has the basic functions of the prescription and reflects the overall curative effect of the prescription. Salvia miltiorrhiza-safflower is a well-known blood-activating drug pair. Salvia miltiorrhiza unblocks the blood and dispels stagnation; The two medicines all enter the heart and liver channels, and the com...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/00
CPCA61K36/286A61K36/537A61K2236/33A61K2236/331A61K2236/333A61K2300/00
Inventor 唐于平瞿城庞汉青段金廒宿树兰
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com