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Composition with salviae miltiorrhiza and safflower as well as preparation method and application thereof

A composition and technology of safflower, a new use in the preparation of antithrombotic disease drugs, the composition of salvia miltiorrhiza and safflower and the field of extraction thereof, can solve the shortage of standard products, and cannot be widely and accurately used for identifying traditional Chinese medicines complex components, expensive detection costs, etc.

Active Publication Date: 2016-09-07
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recently, LC-MS has identified more chemical components through analysis strategies such as the difference in collision energy, characteristic ion filtering, and energy neutral loss. However, these methods are not yet extensive and accurate due to the serious shortage of standards and the high cost of detection. for the identification of complex components of traditional Chinese medicine

Method used

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  • Composition with salviae miltiorrhiza and safflower as well as preparation method and application thereof
  • Composition with salviae miltiorrhiza and safflower as well as preparation method and application thereof
  • Composition with salviae miltiorrhiza and safflower as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0022] 1. Instruments and reagents

[0023] American Waters 2695 high performance liquid chromatography system, Waters 2998PDA detector, Empower data management system, 16 desktop centrifuges (Beckman Coulter, USA), FW80 high-speed universal centrifuge (Tianjin Test Instrument Co., Ltd.), YRE-301 rotary evaporator (Gongyi Yuhua Instrument Co., Ltd.), BT125 electronic balance (Germany Sartorius Scientific Instrument Co., Ltd.), EPED ultrapure water system (Nanjing Epod Technology Development Co., Ltd.).

[0024] Acetonitrile (Scharlab, Spain) was a chromatographically pure reagent, methanol (Nanjing Chemical Reagent Co., Ltd.), and formic acid (Merck, Germany) were analytically pure, and ultrapure water (self-made). Reference substances: salvianolic acid B (DFB, batch number 111562) and tanshinone IIA (DST, batch number 110766) were purchased from China Institute for the Control of Pharmaceutical and Biological Products; hydroxysafflower yellow A (HSYA, batch number 140827) w...

Embodiment 2

[0063] Embodiment 2 antithrombotic comparative experiment

[0064] Effects of Salvia Miltiorrhiza and Safflower in Different Weight Ratio on Coagulation Time

[0065] At present, the conventional method for screening antithrombotic compounds is to investigate the activity of the compound in inhibiting platelet aggregation and the influence on the coagulation time. The present invention examines the antithrombotic activity of each compound by measuring the influence of the compound on the coagulation time.

[0066] Specific method: Take healthy male rabbits, inject 30mg / kg of rabbit body weight pentobarbital sodium saline solution into the ear vein for anesthesia, separate the common carotid artery to take blood, collect it in a plastic centrifuge tube, add 3.8% citric acid Sodium aqueous solution anticoagulant (volume ratio of blood to anticoagulant is 9:1). Centrifuge at 800r / min for 10min to prepare platelet-rich plasma (Platelet-rich plasma, PRP), and centrifuge at 3000r / m...

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Abstract

The invention discloses a composition with salviae miltiorrhiza and safflower. The weight ratio of salviae miltiorrhiza to safflower is (5 to 1) to (5 to 2). Through intensive research on a large number of ancient and modern prescriptions and traditional Chinese medicine application data, active components in salviae miltiorrhiza and safflower are classified according to chemical structure types on the basis of salviae miltiorrhiza and safflower to the existing research; the HPLC-PDA technique is adopted; a chemical substance library is built; a chemical fuzzy recognition strategy is introduced; separated chromatographic peaks of a chromatogram are used for classifying and identifying components and structures according to a characteristic ultraviolet absorption spectrum; dynamic variation rules of different types of chemical components under different preparation methods and different weight ratios are researched by designing symmetry ratios; a weight ratio medicine pair of salviae miltiorrhiza and safflower with optimal weight ratio is screened; the experiment result shows that the preferably-prepared composition with salviae miltiorrhiza and safflower has a good function of resisting cardiovascular and cerebrovascular diseases.

Description

technical field [0001] The invention relates to a traditional Chinese medicine composition, in particular to the composition of salvia miltiorrhiza and safflower, its extraction method and its new application in the preparation of antithrombotic disease medicine. Background technique [0002] "Drug pair" is a commonly used form of compatibility in clinical prescriptions of traditional Chinese medicine, and it is the sublimation of the clinical medication experience accumulated by physicians of all ages. The medicine pair is a bridge connecting a single traditional Chinese medicine and several prescriptions. It is the essence and core of prescription compatibility. It has the basic functions of the prescription and reflects the overall curative effect of the prescription. Salvia miltiorrhiza-safflower is a well-known blood-activating drug pair. Salvia miltiorrhiza unblocks the blood and dispels stagnation; The two medicines all enter the heart and liver channels, and the com...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/537A61P7/02A61P9/10A61P9/00
CPCA61K36/286A61K36/537A61K2236/33A61K2236/331A61K2236/333A61K2300/00
Inventor 唐于平瞿城庞汉青段金廒宿树兰
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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