Application of reagent dose reducing histochemical pen in immunohistochemical detection of cell growing slides

Abstract

The invention discloses application of a reagent dose reducing histochemical pen in immunohistochemical detection of cell growing slides. Specifically, cells are inoculated into a glass slide type culture dish to let cells grow on a glass slide, and immune cell histochemical staining identification is carried out at the end of cell culture. The glass slide type culture dish is provided with a culture dish body and a dish cover, the culture dish body falls into two types, i.e. a single-hole chamber and a porous chamber. The area of each hole chamber is larger than the glass slide, the dish bottom is directly used as a glass slide, the outside of the dish bottom is provided with a rectangular frame trace line dent corresponding to the glass slide size, and under the action of an external force, the dish bottom can get off along with the rectangular frame trace line dent. The histochemical pen is applicable to various immunohistochemical staining experiments of glass slides, can significantly reduce the dosage of antibodies and reagents, avoid liquid flow and diffusion during staining, and improve the operation speed, and is particularly applicable large-scale, large sample and multi-group immunohistochemical staining in the experimental study of cell growing slides or cell smears.

Description

[0001] This application is a divisional application with application number: 201410500701.1, application date: 2014.9.26, and name: "Application of multi-purpose immunohistochemical pen in immunohistochemical detection of cell slides". technical field [0002] The invention relates to the application of an immunohistochemical pen in immunohistochemical detection of cell slides. Background technique [0003] Using specific antibodies to conduct in situ qualitative, localization or quantitative research on the distribution of certain chemical components and their content in tissue slices and cell slides, this technique is called immunocytochemistry (immunocytochemistry) technique. [0004] Usually, the immunohistochemical detection of cell slides is to stain a single cell slide one by one. Generally, only one type of antibody, protein and gene detection can be performed on a single sample, which is difficult for large-scale, multi-sample and multi-group testing. Medical experi...

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Problems solved by technology

[0004] Usually, the immunohistochemical detection of cell slides is to stain a single cell slide one by one. Generally, only one type of antibody, protein and gene detection can be performed on a single sample, which is difficult for large-scale, multi-sample and multi-group testing. Medical Experimental Scientific Research

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