Application of reagent dose reducing histochemical pen in immunohistochemical detection of cell growing slides

An immunohistochemistry and histochemical pen technology, which is applied in the preparation of test samples, biological tests, material inspection products, etc., can solve problems such as incompetence for large-scale, avoid liquid flow and diffusion, and reduce the amount of antibodies and reagents. , the effect of convenient operation

Active Publication Date: 2016-09-14
上海逐典生物科技有限公司
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Problems solved by technology

[0004] Usually, the immunohistochemical detection of cell slides is to stain a single cell slide one by one. Generally, only one type of antibody, protein and gene detection can be performed on a single sample, which is difficult for large-scale, multi-sample and multi-group testing. Medical Experimental Scientific Research

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  • Application of reagent dose reducing histochemical pen in immunohistochemical detection of cell growing slides
  • Application of reagent dose reducing histochemical pen in immunohistochemical detection of cell growing slides
  • Application of reagent dose reducing histochemical pen in immunohistochemical detection of cell growing slides

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Embodiment Construction

[0038] An application of a multipurpose immunohistochemical pen in the immunohistochemical detection of cell slides, cells are inoculated in a glass slide culture dish for cell slides, and immunocytochemical staining is carried out after the cell culture is completed; the said The slide-type petri dish is equipped with a petri dish body 1 and a dish cover 2. The petri dish body is divided into two types: single-hole chamber and multi-hole chamber; the area of ​​each hole chamber is larger than the slide glass, and the bottom 3 of the dish body is directly used as a glass slide , the outer surface of the bottom of the dish body is provided with a rectangular frame trace line groove 4 corresponding to the size of the glass slide, and the bottom of the dish body can be pulled out along the rectangular frame trace line groove under the action of an external force;

[0039] The immunocytochemical staining identification includes the following steps in turn:

[0040] (1) Fix with ic...

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Abstract

The invention discloses application of a reagent dose reducing histochemical pen in immunohistochemical detection of cell growing slides. Specifically, cells are inoculated into a glass slide type culture dish to let cells grow on a glass slide, and immune cell histochemical staining identification is carried out at the end of cell culture. The glass slide type culture dish is provided with a culture dish body and a dish cover, the culture dish body falls into two types, i.e. a single-hole chamber and a porous chamber. The area of each hole chamber is larger than the glass slide, the dish bottom is directly used as a glass slide, the outside of the dish bottom is provided with a rectangular frame trace line dent corresponding to the glass slide size, and under the action of an external force, the dish bottom can get off along with the rectangular frame trace line dent. The histochemical pen is applicable to various immunohistochemical staining experiments of glass slides, can significantly reduce the dosage of antibodies and reagents, avoid liquid flow and diffusion during staining, and improve the operation speed, and is particularly applicable large-scale, large sample and multi-group immunohistochemical staining in the experimental study of cell growing slides or cell smears.

Description

[0001] This application is a divisional application with application number: 201410500701.1, application date: 2014.9.26, and name: "Application of multi-purpose immunohistochemical pen in immunohistochemical detection of cell slides". technical field [0002] The invention relates to the application of an immunohistochemical pen in immunohistochemical detection of cell slides. Background technique [0003] Using specific antibodies to conduct in situ qualitative, localization or quantitative research on the distribution of certain chemical components and their content in tissue slices and cell slides, this technique is called immunocytochemistry (immunocytochemistry) technique. [0004] Usually, the immunohistochemical detection of cell slides is to stain a single cell slide one by one. Generally, only one type of antibody, protein and gene detection can be performed on a single sample, which is difficult for large-scale, multi-sample and multi-group testing. Medical experi...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N1/31G01N33/53
CPCG01N1/30G01N1/31G01N33/53
Inventor 江明张冲鄂群曹靖晨
Owner 上海逐典生物科技有限公司
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