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Establishment method of cLYZ/hTLF double-antibacterial gene recombinant adenovirus, and recombinant adenovirus and application thereof

A technology of recombinant adenovirus and construction method, applied in the field of construction of recombinant adenovirus, can solve problems such as difficulty in the treatment of bacterial drug-resistant endemic dairy cow mastitis

Active Publication Date: 2016-10-12
TIANJIN AGRICULTURE COLLEGE
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Problems solved by technology

At present, the prevention and treatment of the disease is mainly through standardized breeding management and the use of antibiotics and chemotherapy drugs. Difficulties in the treatment of endemic dairy cow mastitis

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  • Establishment method of cLYZ/hTLF double-antibacterial gene recombinant adenovirus, and recombinant adenovirus and application thereof
  • Establishment method of cLYZ/hTLF double-antibacterial gene recombinant adenovirus, and recombinant adenovirus and application thereof
  • Establishment method of cLYZ/hTLF double-antibacterial gene recombinant adenovirus, and recombinant adenovirus and application thereof

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Embodiment Construction

[0021] The present invention will be further described in detail below in conjunction with the drawings and specific embodiments:

[0022] With the development of molecular biology, immunology, pathology and related disciplines, gene therapy has become a hot spot in disease prevention research. At present, adenovirus (Ad) vector therapy is one of the most promising gene transfer methods in gene therapy. It can effectively deliver foreign genes to various target cells or tissues. The vector construction and operation are simple. The host has a wide range, strong infectivity, does not integrate into the host chromosome, and can infect and express multiple target genes in proliferating and non-proliferating cells.

[0023] Fish are the most primitive and lowest level group in the vertebrate subphylum. Although they have specific and non-specific immune systems, their specific immune systems are underdeveloped compared with mammals. Therefore, the non-specific immune system of fish is...

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Abstract

The invention discloses an establishment method of a cLYZ / hTLF double-antibacterial gene recombinant adenovirus, and a recombinant adenovirus and application thereof. The method comprises the following steps: respectively amplifying cLYZ gene and hTLF gene from grouper head-kidney tissues and human milk somatic cell total RNAs, cloning the obtained goal genes into a pDC315 shuttle vector, co-transfecting HEK293 cells through liposome by using the target-gene-containing shuttle plasmid and adenovirus framework plasmid, and packaging to obtain the high-titer recombinant adenovirus; and evaluating the combined inhibiting actions of the cLYZ gene and hTLF gene on the milk cow mastitis primary pathogens under the adenovirus mediated conditions.

Description

Technical field [0001] The invention relates to a method for constructing a recombinant adenovirus, in particular to a method for constructing a recombinant adenovirus with cLYZ and hLTF double antibacterial genes, as well as a recombinant adenovirus and application. Background technique [0002] Dairy cow mastitis, also known as cow mastitis, is an inflammatory reaction that occurs when the mammary glands of cows are exposed to external stimuli or infected by pathogenic bacteria. It will not only cause a decrease in milk production of dairy cows, shorten the replacement cycle of dairy cows, and even cause complete loss of lactation ability of sick dairy cows in severe cases. Therefore, the clinical prevention and treatment of dairy cow mastitis is a major problem that has always plagued the dairy industry. At present, the prevention and treatment of the disease is mainly through the introduction of standardized breeding management and the use of antibiotics and chemotherapeutic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/01C12N15/861A61K38/47A61K38/40A61P31/04C12R1/93
CPCC07K14/79C12N7/00C12N9/2462C12N15/86C12N2710/10021C12N2710/10043C12N2800/107C12Y302/01017
Inventor 金天明弓建芳张东超李小慧高珂珂林静孟佳丽尚翠玲李昕
Owner TIANJIN AGRICULTURE COLLEGE
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