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A biomimetic magnetosome loaded with siRNA and its preparation method

A magnetosome, biomimetic technology, applied in the fields of chemistry and biomedicine, can solve problems such as cytotoxicity

Inactive Publication Date: 2019-08-30
BEIJING INSTITUTE OF TECHNOLOGYGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The long cycle inside the biological organism can be realized by modifying or modifying the surface of the carrier system. At present, most of the surface modification, such as polyethylene glycol (polyethylene glycol, PEG) or other modifications, can realize the carrier inside the biological organism. The long cycle, although the cycle time is prolonged, but most of them are cytotoxic

Method used

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  • A biomimetic magnetosome loaded with siRNA and its preparation method
  • A biomimetic magnetosome loaded with siRNA and its preparation method
  • A biomimetic magnetosome loaded with siRNA and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0123] A method for preparing a biomimetic magnetosome loaded with siRNA, the specific steps of the method are as follows:

[0124] Step 1, preparation of MNC

[0125] (1) Put DEG in the environment of Ar gas, after the oxygen is removed completely, dissolve the solid NaOH into the DEG, heat at the same time, raise the temperature to 120°C, stop heating until the solid NaOH is completely dissolved, the whole preparation process uses Protected by Ar gas, a light yellow solution was prepared, which was a NaOH storage solution, kept at 70°C for future use;

[0126] (2) FeSO 4 ·7H 2 O, PEI and solvent are mixed uniformly and vacuumized for 25min, then filled with Ar gas protection; heating up to 160°C, adding the NaOH storage solution obtained in step 1 (1), until the molar concentration of NaOH in the solution is 0.03mol / L, about After 3 minutes, the color of the solution turned black, and then the temperature was raised to 220 ° C, and the reflux was continued for 1 hour, and...

Embodiment 2

[0189] A method for preparing a biomimetic magnetosome loaded with siRNA, the specific steps of the method are as follows:

[0190] Step 1, preparation of MNC

[0191] (1) Put DEG in the environment of Ar gas, after the oxygen is removed completely, dissolve the solid NaOH into the DEG, heat at the same time, raise the temperature to 100°C, stop heating until the solid NaOH is completely dissolved, the whole preparation process uses Protected by Ar gas, a light yellow solution was prepared, which was a NaOH storage solution, kept at 70°C for future use;

[0192] (2) FeCl 2 4H 2 O, PEI and solvent are evenly mixed and vacuumed for 25min, then filled with Ar gas protection; heating to 100°C, adding the NaOH storage solution obtained in step 1 (1), until the molar concentration of NaOH in the solution is 0.03mol / L, about After 3 minutes, the color of the solution turned black, and then the temperature was raised to 190°C, and reflux was continued for 30 minutes, and the reacti...

Embodiment 3

[0209] A method for preparing a biomimetic magnetosome loaded with siRNA, the specific steps of the method are as follows:

[0210] Step 1, preparation of MNC

[0211] (1) Put the DEG in the environment of Ar gas, after the oxygen is completely removed, dissolve the solid NaOH into the DEG, heat it at the same time, raise the temperature to 150°C, and stop heating until the solid NaOH is completely dissolved. The whole preparation process uses Protected by Ar gas, a light yellow solution was prepared, which was a NaOH storage solution, kept at 70°C for future use;

[0212] (2) FeSO 4 ·7H 2 O, PEI and solvent are evenly mixed and then vacuumed for 25min, then filled with Ar gas protection; heating up to 180°C, adding the NaOH storage solution obtained in step 1 (1), until the molar concentration of NaOH in the solution is 0.4mol / L, about After 3 minutes, the color of the solution turned black, and then the temperature was raised to 230°C, and continued to condense and reflux...

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Abstract

The invention relates to a bionic magnetosome loaded with siRNA and a preparation method of the magnetosome, and belongs to the field of chemistry and biomedicine. According to the bionic magnetosome, water-soluble MNC serves as the kernel and is wrapped by a white cell membrane, and siRNA is loaded between the MNC and the white cell membrane; antibodies or peptides with modifying groups can be coupled outside the white cell membrane of the bionic magnetosome in a covalence mode. By preparing MNC, M and MNC:siRNA and mixing an MNC:siRNA solution, an M solution and a buffer solution, the bionic magnetosome is obtained; the white cell membrane of the bionic magnetosome is modified by the peptides or antibodies through the modifying groups, and the bionic magnetosome coupled with the antibodies or peptides is obtained. The MNC in the bionic magnetosome has positive charges, can adsorb siRNA and is wrapped by M for bionics, a focus is reached efficiently and rapidly, and meanwhile immune elimination of organisms is avoided.

Description

technical field [0001] The invention relates to a biomimetic magnetosome loaded with siRNA and a preparation method thereof, belonging to the fields of chemistry and biomedicine. Background technique [0002] RNA interference (RNAi) refers to the specific gene silencing after inducing transcription of exogenous or endogenous double-stranded small RNA (siRNA) homologous to the target gene in organism cells. RNAi drugs have shown great application prospects in tumors, genetic diseases and other diseases, but so far, RNAi technology still has siRNA targeting and off-target effects, in vivo instability, interferon effects, and drug delivery in the treatment of tumors. System, delivery method and drug safety and other issues. [0003] Nanocarrier-mediated gene transfection has advantages that other drug carriers do not have, especially magnetic nanoparticles have good biocompatibility, superior magnetic properties and high stability, and can be used for magnetically targeted siR...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K31/713A61K47/58A61K47/46A61K47/34A61K41/00A61P35/00
Inventor 谢海燕张帆马光辉魏炜赵龙剑
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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