Animal learning memory individualized evaluating method based on water maze

A technology of learning and memory and evaluation method, applied in the field of individualized evaluation of animal learning and memory based on water maze, can solve the problems of lack of pertinence, lack of scientificity, individual evaluation of animals, etc. scientific effect

Active Publication Date: 2016-11-09
HARBIN UNIV OF COMMERCE
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

At the same time, because the traditional evaluation method assumes that the incidence of cognitive impairment in animals is 100%, if the use of drug intervention therapy is not targeted, a part of the normal animals are also included in the evaluation of drug efficacy.
Therefore, the traditional data analysis method has shortcomings such as insufficient scientificity and the inability to perform individual evaluation on animals.

Method used

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  • Animal learning memory individualized evaluating method based on water maze
  • Animal learning memory individualized evaluating method based on water maze
  • Animal learning memory individualized evaluating method based on water maze

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Experimental program
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Effect test

Embodiment 1

[0015] The individualized evaluation method of animal learning and memory based on the water maze implemented in this implementation is a study on the individual cognitive function of normal ICR mice. The research process is as follows:

[0016] 20 ICR mice weighing 20±2g, 4-5 weeks old, half male and half male, were selected for Morris water maze experiment. If the traditional statistical method is adopted, this group is a blank control group, and the mice with cognitive impairment in this group are assumed to be 0% by default. However, using this evaluation method, it was found that the incubation period of the ICR mice for the third training was 94.8s, and during the period from the sixth to the tenth training, there were 2 mice with abnormal cognitive function, and the incidence rate of cognitive function was 10.0%. (The results are shown in Table 1). Compared with the traditional statistical method, this data is more in line with the actual law, and the evaluation method ...

Embodiment 2

[0020] The individualized evaluation method of animal learning and memory based on the water maze implemented in this implementation is the individual cognitive function research of normal Wistar rats, and the research process is as follows:

[0021] Select 20 Wistar rats weighing 200±20g, 6-8 weeks old, half male and half male, for Morris water maze experiment. If the traditional statistical method is used, this group is the blank control group, and the rats with cognitive impairment in this group are assumed to be 0% by default. However, using this evaluation method, it was found that the incubation period of Wistar rats was 58.0s for the third time of training, and there was only one mouse with abnormal cognitive function during the period from the sixth to the tenth time of training, and the incidence rate of cognitive function was 5.0%. (The results are shown in Table 2). Compared with the traditional statistical method, this data is more in line with the actual law, and ...

Embodiment 3

[0025] The individualized evaluation method of animal learning and memory based on water maze implemented in this implementation is the study of individual cognitive function of mice injected with scopolamine. The research process is as follows:

[0026] The cognitive impairment model was established by intraperitoneal injection of scopolamine. Select 20 mice weighing 20±2g, 4-5 weeks old, half male and half male, and inject scopolamine solution 1 mg / kg intraperitoneally 20 minutes before the water maze test to establish a dementia mouse model. If the traditional statistical method is used, this group is the model control group, and the mice with cognitive impairment in this group are assumed to be 100% by default. However, using this evaluation method, it was found that from the 6th to the 10th time of training, there were 16 mice with abnormal cognitive function, and the incidence rate of cognitive function was 80.0% (results are shown in Table 3), compared with the traditio...

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Abstract

The invention discloses an animal learning memory individualized evaluating method based on a water maze. The animal individualized evaluating method is used for determining cognition abnormity by analyzing data of the time (incubation period) from water entering to platform boarding of multiple batches of mice / rats with a Morris water maze experiment as a tool and normal animals and cognition abnormal animals as the objects. Compared with a traditional method emphasizing comparison among groups of samples, the method has the advantages that the overgeneralization means of the pure 'average value + / - standard deviation' form in the traditional method is abandoned, and model animals are separated according to the cognition difference and studied one by one; by means of the method, the animal cognition level difference can be more objectively reflected, and the state is closer to the real state of animals; the method is easy to operate and feasible, and the reliability and scientificity of the method are ensured through repeated experiments of multiple batches of animals and the confirmatory experiments of models.

Description

technical field [0001] The invention relates to a method for individualized evaluation of animal learning and memory based on a water maze Background technique [0002] The Morris water maze is currently recognized as a testing device for evaluating the learning and memory functions of rats and mice. It is widely used for its advantages of eliminating odor interference, environmental interference, short experimental period, and no need for fasting. However, it also has disadvantages such as redundant data and cumbersome processing. At present, the data processing method of Morris water maze mostly adopts the traditional statistical method, that is, under the premise of 0% cognitive impairment in the default blank control group, compare the model group with the control group, and compare the significant difference between the two. This approach is equal to the default incidence rate of cognitive impairment as 100%. Although it conforms to the rules of mathematical statistics...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 赵瑛刘琳刘月婷余群星
Owner HARBIN UNIV OF COMMERCE
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