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Method for identifying salvia miltiorrhiza in bone-pain medical wine

A technology of bone pain medicinal wine and Danshen, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as difficult to effectively control the quality of medicines, and achieve the effects of improving quality inspection standards, strong controllability, and high sensitivity

Inactive Publication Date: 2016-11-23
GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing quality standards for bone pain medicinal liquor, the content is only the routine inspection items of the liquor, so it is difficult to effectively control the quality of the medicine

Method used

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  • Method for identifying salvia miltiorrhiza in bone-pain medical wine
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  • Method for identifying salvia miltiorrhiza in bone-pain medical wine

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Experimental program
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Effect test

Embodiment 1

[0048] The identification method of salvia miltiorrhiza in bone pain medicinal wine comprises the following steps:

[0049] (1) Preparation of the test solution: take 95 mL of this product, add 20 mL of chloroform each time, extract 4 times, combine the chloroform solution, evaporate to dryness, add 1 mL of ethyl acetate to the residue to dissolve it, and use it as the test sample solution;

[0050] (2) Preparation of reference substance solution: take tanshinone Ⅱ A reference substance, add ethyl acetate to make a solution containing 2 mg of tanshinone Ⅱ A reference substance per 1 mL, as the reference substance solution;

[0051] (3) Identification: According to the "Chinese Pharmacopoeia" 2015 edition four general rules 0502 thin-layer chromatography test, absorb 8 μL of the reference substance solution and 8 μL of the test solution, respectively spot on the same silica gel G thin-layer plate, with benzene-ethyl acetate =18︰1 is the developer, develop, take out, and dry, i...

Embodiment 2

[0053] The identification method of salvia miltiorrhiza in bone pain medicinal wine comprises the following steps:

[0054] (1) Preparation of the test solution: take 105 mL of this product, add 30 mL of chloroform each time, extract twice, combine the chloroform solution, evaporate to dryness, add 2 mL of ethyl acetate to the residue to dissolve it, and use it as the test product solution;

[0055] (2) Preparation of reference substance solution: take tanshinone Ⅱ A reference substance, add ethyl acetate to make a solution containing 2 mg of tanshinone Ⅱ A reference substance per 1 mL, as the reference substance solution;

[0056] (3) Identification: According to the TLC test of "Chinese Pharmacopoeia" in 2015, the four general rules 0502, draw 12 μL of the reference substance solution and 12 μL of the test solution, respectively spot on the same silica gel G thin-layer plate, and use benzene-ethyl acetate =25︰2 is the developing agent, develop, take out, and dry, in the chr...

Embodiment 3

[0058] The identification method of salvia miltiorrhiza in bone pain medicinal wine comprises the following steps:

[0059] (1) Preparation of the test solution: Take 100 mL of this product, add 15 mL of chloroform each time, extract 3 times, combine the chloroform solution, evaporate to dryness, add 0.5 mL of ethyl acetate to the residue to dissolve it, and use it as the test solution. product solution;

[0060] (2) Preparation of reference substance solution: take tanshinone Ⅱ A reference substance, add ethyl acetate to make a solution containing 2 mg of tanshinone Ⅱ A reference substance per 1 mL, as the reference substance solution;

[0061] (3) Identification: According to the "Chinese Pharmacopoeia" 2015 edition four general rules 0502 thin-layer chromatography test, absorb 10 μL of the reference substance solution and 10 μL of the test solution, respectively spot on the same silica gel G thin-layer plate, with benzene-ethyl acetate =19︰1 is the developing agent, develo...

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Abstract

The invention discloses a method for identifying salvia miltiorrhiza in bone-pain medical wine. The method is a thin layer chromatography in which a tanshinone IIA reference substance is taken as a contrast, and benzene and ethyl acetate in a ratio of (18-25) to (1-2) are taken as a developing solvent. The method has the beneficial effects that the operation is simple, the controllability is strong, and the salvia miltiorrhiza component in the bone-pain medical wine can be accurately identified; and the separation degree, the sensitivity and the precision are high, the stability and the reproducibility are good, the interference of a negative control is avoided, and the resultes are not influenced by thin layer plates of different manufacturers and the temperature and humidity conditions. According to the method, the salvia miltiorrhiza component in the bone-pain medical wine can be accurately, comprehensively and scientifically identified, so that the quality test standard is improved, and the stability, safety and effectivity of the product quality are further guaranteed.

Description

technical field [0001] The invention relates to a method for identifying salvia miltiorrhiza in bone pain medicinal wine, and specifically belongs to the technical field of traditional Chinese medicine detection. Background technique [0002] Bone Pain Medicinal Wine is made from Aconitum Aconitum, Sangji, Aescinus, Clematis, Polygonum cuspidatum, Caterpillar, Sarsaparilla, Cangzhu (stir-fried with bran), Chinese pine knots, Polygonum multiflorum, Red vine, Salvia miltiorrhiza, Elderberry, It is made of Shenjincao, papaya, Chuanxiong, Achyranthes bidentata, ephedra, Xiangjia bark, safflower, Dipsacus and dried ginger. Bone Pain Medicinal Wine has the functions of dispelling wind and relieving pain, relaxing tendons and activating collaterals. The medicine takes advantage of the power of the wine, and the wine can help the medicine, which can quickly open up the healing resistance, unblock the blood vessels, meridians, dissolve the blood stasis on the cartilage, and divide th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 汪尚游
Owner GUIZHOU XINBANG PHARMACEUTICAL CO LTD
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