Kit capable of quickly and quantitatively detecting hog cholera antibody and preparation method of kit

Abstract

The invention relates to a kit for rapid quantitative detection of swine fever antibody. The kit includes a colloidal gold immunochromatography test paper card and a standard curve information card. The colloidal gold immunochromatography test paper card is provided with colloidal gold-labeled hog fever E2-E0 The fusion expression antigen, the colloidal gold-labeled classical swine fever E2-E0 fusion expression antigen is synthesized by connecting the classical swine fever E2-E0 fusion expression antigen and a certain amount of colloidal gold particle solution through physical adsorption, and the colloidal gold particle solution contains The average particle size of colloidal gold is 30-35nm. After the sample to be tested is added to the colloidal gold immunochromatography test paper card, use a color analyzer to read the optical density values ​​of the detection zone and the accusation zone of the test paper card, and then calculate through software according to the standard curve recorded on the standard curve information card. The exact content of swine fever antibody. The invention is easy to operate and produces results quickly, and is a very convenient and good tool for accurately evaluating the immune status of swine fever.

Description

【Technical field】 [0001] The invention relates to the technical field of swine fever antibodies, in particular to a kit for rapid and quantitative detection of swine fever antibodies, which is used for detecting swine fever antibodies in pig serum or plasma samples, and belongs to colloidal gold chromatography immunoassay. 【Background technique】 [0002] Classical swine fever (CSF) is a highly contagious disease caused by swine fever virus (CSFV), which causes serious harm to the pig industry and is a notifiable disease determined by the World Organization for Animal Health (OIE). one of diseases). For many years, CSF has also endangered the development of my country's pig industry. It is one of the four major animal diseases in my country and is legally classified as a first-class infectious disease by the Ministry of Agriculture of my country. CSF is an acute and severe infectious disease with a mortality rate as high as 80% to 90%. Vaccination is the only effective measu...

AI Technical Summary

Problems solved by technology

Because this experiment needs to cultivate infectious swine fever virus, it is difficult for laboratories without its qualifications to carry out this experiment

At the same time, this test requires extremely high professional quality and experience of operators, and is expensive. Only a few qualified laboratories can carry out this test, and it is impossible to promote it in the market.

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