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Method for establishing hyperlipidaemia rat model in short term

A rat model and hyperlipidemia technology, applied in the field of experimental animals, can solve the problems of high cost, decreased volatility of serum total cholesterol, and long cycle, and achieve low cost, short cycle, and good reproducibility

Active Publication Date: 2016-12-21
ZHEJIANG GONGSHANG UNIVERSITY
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Problems solved by technology

However, at present, it generally takes 6-8 weeks to raise a rat model of hyperlipidemia by feeding it with high-fat feed

Method used

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  • Method for establishing hyperlipidaemia rat model in short term
  • Method for establishing hyperlipidaemia rat model in short term
  • Method for establishing hyperlipidaemia rat model in short term

Examples

Experimental program
Comparison scheme
Effect test

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[0030] Example 1 Determination of rat growth performance

[0031] During the rearing period, the rats in each group were weighed every day, the average weight of each group of rats was calculated and recorded, the weight growth curve was drawn, and the weight gain percentage of the rats during the rearing period was calculated. Throughout the experimental period (21 days), with the extension of the feeding time, the weight of the rats in the control group and the model group showed an increasing trend; during the first to 14 days of the experiment, the weight of the two groups of rats maintained a relatively consistent increase Speed; but starting from the 14th day, the weight of the rats in the control group continued to increase at the original speed; while the weight increase of the rats in the model group began to slow down ( figure 1 ). According to the average body weight of the rats throughout the test period, there was no significant difference in the average body weight ...

Example Embodiment

[0034] Example 2 Detection of total cholesterol concentration in rat serum

[0035] During the rearing period, on the 10th, 14th and 21st days of rearing, the blood of each group of rats was collected by the retro-orbital venous plexus blood collection method. After standing for coagulation, the blood was centrifuged at 3000-5000r / min for 10 minutes, and the upper serum was placed The other centrifuge tube is frozen and stored for testing; according to the instructions of the total cholesterol in the serum, the absorbance of each serum sample is detected by the biochemical method on the automatic biochemical analyzer, and the total cholesterol concentration in the sample is calculated according to the formula. by figure 2 It can be seen that in the rat serum taken on the 10th, 14th and 21st days, the total cholesterol concentration in the serum of the model group was significantly higher than that of the control group; on the 10th, 14th and 21st days, the model The concentration...

Example Embodiment

[0036] Example 3 Detection of triglyceride concentration in rat serum

[0037] According to the instructions of the kit for determination of triglycerides in serum, the absorbance of each serum sample was detected on an automatic biochemical analyzer using a biochemical method, and the triglyceride concentration in the sample was calculated according to the formula. by image 3 It can be seen that in the rat serum taken on the 10th, 14th, and 21st days, the triglyceride concentration in the serum of the model group was significantly higher than that of the control group; on the 10th, 14th and 21st days, The serum triglyceride concentration of rats in the model group was increased by 80%, 81%, and 98% respectively compared with the control rats.

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Abstract

The invention discloses a method for establishing a hyperlipidaemia rat model in a short term. Animals are male Sprague Dawley rats, the body weight of the rats is 150+/-20 grams. A high-fat diet formula includes 23.31% of casein, 0.35% of L-cystine, 8.48% of corn starch, 11.65% of maltodextrin, 20.17% of saccharose, 5.83% of cellulose, 2.91% of soybean oil, 20.68% of lard oil, 5.23% of compound mineral substances, 1.16% of compound vitamin, and 0.23% of Choline bitartrate. A fat emulsion formula includes 20% of Tween 80, 20% of lard oil, 10% of cholesterol, 2% of sodium cholate, 20% of propylene glycol, 20% of glucose, and 1% of propylthiouracil. The operation method includes: constinuously feeding rats with the high-fat diet for 21 days, and performing self-feeding; feeding the rats with the fat emulsion prepared according to the formula through the gavage method, wherein the dosage is 2 ml/kg. day. The method is short in establishment cycle of the rat hyperlipidaemia animal model, is low in cost, is good in reproducibility, can effectively simulate the human incidence state. Accordingly, the method can be used for research on functional foods capable of preventing hyperlipidaemia and screening of lipid-lowering drugs.

Description

Technical field [0001] The invention belongs to the technical field of experimental animals, and specifically relates to a method for making rats have pathological characteristics of hyperlipidemia in a short period of time; the model can be used for the research of functional foods for preventing hyperlipidemia, and can also be used for the screening of drugs for reducing blood lipid , Efficacy evaluation and pharmacological research and other fields. Background technique [0002] With the changes in people's diet, the incidence of hyperlipidemia is increasing year by year. Hyperlipidemia is a systemic disease with abnormal lipid metabolism or functioning, that is, the increase in serum total cholesterol, triglycerides, low-density lipoprotein, and the decrease in high-density lipoprotein. It is a sign of lipid metabolism disorder and also an artery Atherosclerosis, heart disease, stroke and fatty liver are one of the main causes that seriously threaten human health. Finding s...

Claims

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 韩菲菲韩剑众范寒雪刘芳
Owner ZHEJIANG GONGSHANG UNIVERSITY
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