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Cladosporium cucumerinum assay kit and detection method thereof

A detection kit and technology of melon blight bacteria, applied in the direction of microorganism-based methods, microorganism measurement/inspection, biochemical equipment and methods, etc., can solve problems such as timely and effective prevention and control difficulties, reduce economic losses, overcome Long time, the effect of disease control

Inactive Publication Date: 2010-12-08
EAST CHINA UNIV OF SCI & TECH +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is difficult to identify the three diseases from the symptoms in the initial stage, so it brings difficulties to timely and effective prevention and treatment.

Method used

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  • Cladosporium cucumerinum assay kit and detection method thereof
  • Cladosporium cucumerinum assay kit and detection method thereof
  • Cladosporium cucumerinum assay kit and detection method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment

[0054] 1 Material method:

[0055] 1.1 Test strains:

[0056] The species name, source and quantity of the tested strains used for screening with specific primers in this embodiment are shown in the table below.

[0057] Table 1

[0058]

[0059]

[0060] Note: + means amplified product; - means no amplified product.

[0061] 1.2 Mycelium cultivation and collection

[0062] Cucumber scab bacterium for testing is on the PDA flat plate, after 21 ℃ of cultivations for 5-7 days, the mycelium pieces are cut from the edge of the colony and transferred to the PDB liquid medium (potato dextrose liquid medium) (in every 250ml Erlenmeyer flask) 100ml culture solution), 21°C, 100rpm shaking culture for 7 days, filter to collect mycelia, freeze-dry and grind into mycelium powder, and store at -20°C for future use.

[0063] On the PDA (potato dextrose agar medium) plate, the watermelon wilt bacteria and watermelon wilt bacteria for testing were cultivated for 7 days at 26°C, and ...

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Abstract

The invention provides primer composition used for specifically detecting cladosporium cucumerinum and / or fusarium oxysporum and mycosphaerella melonis in sample and a PCR assay kit containing the primer composition. The invention also provide a method for detecting cladosporium cucumerinum and / or fusarium oxysporum and mycosphaerella melonis in sample. The method of the invention not only can rapidly, simply and accurately detect disease, and detection cost can be greatly saved.

Description

technical field [0001] The present application relates to the field of biotechnology, especially the field of biological detection. Specifically, the application relates to a detection kit and a detection method for Melon scab bacteria. Background technique [0002] Melon scab (Cladosporium cucumerinum, also known as melon scab) can cause cucumber, watermelon, zucchini, pumpkin, muskmelon, winter melon and other melon diseases, and scab on cucumber is the most common [1]. Melon scab is a devastating disease on melons. Due to its serious damage and difficult control, it has been listed as a national plant quarantine pest in my country. Fusarium oxysporum and Didymelabryoniae (Mycosphaerella meloni in domestic reports) are also important pathogens of melons. These three pathogens are very common in melons. [0003] Cucumber scab can be infected at the seedling stage and adult plant stage. The pathogenic bacteria can infect young leaves, tender stems and young melons. It is a...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12R1/77C12R1/645
Inventor 王伟高永洋王楠高观朋唐建辉
Owner EAST CHINA UNIV OF SCI & TECH
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