Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for rapidly identifying Fraxinus velutina and Fraxinus pennsylvanica hybrid by using SSR (simple sequence repeat) marking technology

A technology of P. fluff and labeling technology, which is applied in the directions of biochemical equipment and methods, determination/inspection of microorganisms, etc., and can solve the problem of rapid identification of P. fluff and American red ash hybrids, rapid identification of unfavorable hybrids, and unstable morphological characteristics. and other problems, to achieve the effect of clear amplification bands, improved breeding efficiency, rapid accuracy and authenticity

Active Publication Date: 2016-12-21
SHANDONG FOREST SCI RES INST
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phenotypic traits are important morphological markers for identifying hybrids, but it is time-consuming and laborious, and many morphological characteristics are unstable due to environmental and other factors, which is not conducive to the rapid identification of hybrids
However, after retrieval, there is no rapid identification method for hybrids of ash and American red ash using SSR marker technology at home and abroad

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly identifying Fraxinus velutina and Fraxinus pennsylvanica hybrid by using SSR (simple sequence repeat) marking technology
  • Method for rapidly identifying Fraxinus velutina and Fraxinus pennsylvanica hybrid by using SSR (simple sequence repeat) marking technology
  • Method for rapidly identifying Fraxinus velutina and Fraxinus pennsylvanica hybrid by using SSR (simple sequence repeat) marking technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038] The present invention will be described in detail below in conjunction with specific embodiments.

[0039] 1. Materials and methods

[0040] 1. Materials

[0041] The parents of Chinese red ash "Luwa No. 3", American red ash "Luwa No. 5" and their hybrid plants were used as materials.

[0042] 2. Genomic DNA extraction

[0043] Extract according to the steps of the Plant Genomic DNA Extraction Kit (spin column type, trademark number DP305-03) produced by TIANGEN:

[0044] 1) Take about 100mg of fresh plant tissue or about 30mg of dry weight tissue, add liquid nitrogen and grind thoroughly;

[0045]2) Quickly transfer the ground powder to a centrifuge tube pre-installed with 700ul 65°C preheated buffer GP1 (add mercaptoethanol to the preheated GP1 before the experiment to make the final concentration 0.1%), and quickly invert to mix After homogenization, place the centrifuge tube in a 65°C water bath for 20 minutes, and invert the centrifuge tube during the water bat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for rapidly identifying a Fraxinus velutina and Fraxinus pennsylvanica hybrid by using SSR (simple sequence repeat) marking technology. Genome DNA of Fraxinus velutina and Fraxinus pennsylvanica parents and their hybrid is used as a template, and an SSR primer is designed by using a constructed Fraxinus velutina transcriptome database; SSR markers with high, stable and clear polymorphism and parent complementary bands are screened out, hybrid plants are identified to obtain a pair of suitable SSR markers, the markers amplify a special band identical with a female parent from hybrid offspring, and hybrids having the female parent special band are true hybrids. The method enables quick and accurate identification for the authenticity of Fraxinus velutina and Fraxinus pennsylvanica hybrids, and good basis is laid for identifying hybrids of different combinations of Fraxinus parents.

Description

technical field [0001] The invention belongs to the technical field of identification of plant hybrids, and in particular relates to a method for quickly identifying hybrids of white wax tomentosa and American red ash by using SSR marking technology. Background technique [0002] F. velutina Torr. and F. pennsylvanica are deciduous trees of the Oleaceae family (Oleaceae), which are successfully introduced from the United States in my country. Because of their wind resistance, smoke resistance, and salt tolerance Alkaline and ornamental, it is an important tree species for saline-alkali land afforestation and urban landscaping in my country. Due to their great advantages in adaptability and ornamental properties, these imported species of Ashes soon occupied a pivotal position in northern my country, especially in saline-alkali land afforestation, and their breeding work began immediately. However, the research progress on species improvement of Ash species is slow, and the m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/156
Inventor 燕丽萍吴德军王因花刘翠兰臧真荣李庆国姚俊修任飞
Owner SHANDONG FOREST SCI RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com