DC cell membrane bionic liposome drug carrier as well as preparation method and application thereof

Abstract

The invention relates to a targeting personalized DC cell membrane bionic liposome drug carrier as well as a preparation method and application thereof. The DC cell membrane bionic liposome drug carrier is characterized in that (1) the liposome drug carrier has a cell membrane protein component; (2) the liposome drug carrier can carry a drug in vitro and is used for cell targeting fused release; and (3) the cell membrane protein component of the liposome comes from immortalized somatic cells. The invention has the following advantages: the drug carrier can be applied to the targeting delivery to in-vivo DC cells of a drug or antigen, the antigen presentation ability of the DC cells is enhanced, and the specific immunity is improved.

Description

technical field [0001] The invention belongs to the technical field of cell therapy and precise delivery of liposome drugs, relates to a DC cell membrane bionic liposome drug carrier, also relates to a preparation method of the above cell membrane bionic liposome drug carrier, and also relates to a cell membrane bionic drug carrier Application of liposomal drug carriers for precise targeting of dendritic cells (DCs) in vivo. Background technique [0002] Current targeted drug delivery systems include liposomes, emulsions, microspheres, nanoparticles, etc., wherein the liposome drug delivery system is loaded with drugs in ultramicrospherical vesicles encapsulated by lipid molecules (liposomes) In the middle, the interior is a water-based space, which can be loaded with hydrophilic drugs, and the outer layer of lipid molecules is a space for lipophilic drugs. The size is generally several nm to several μm, and it is divided into passive and active targeting liposomes. . At p...

AI Technical Summary

Problems solved by technology

However, the production of traditional liposomes has not paid enough attention to cell membrane protein biomimetic liposomes. First, the types of membrane receptors and membrane fusion-related proteins on the surface of different types of cells are different, and it is difficult to apply universally. They are some cell-specific membrane fusion proteins; moreover, there are many types of proteins involved in cell membrane fusion, and it is not easy to do the separation of related proteins and the integration of liposome membranes; because most of the membrane proteins pass through the endoplasmic reticulum and Golgi apparatus Glycosylation modification. Recombinant proteins generally lack these glycosylation modifications. Yeast-expressed proteins have excessive glycosylation, and there is a risk of antibody production when using yeast-derived recombinant proteins, and the recombinant expression of various cell membrane proteins will also be a relatively difficult task. Large workload, the above-mentioned difficulties have hindered people from developing the development of cell membrane biomimetic liposomes.

Images