Formula of tissue fixing liquid for coloring tissue and usage method thereof
A technology of tissue fixation and formulation, applied in the preparation of test samples, etc., can solve the problems of eosin color fading, inability to find and identify small specimens, and lightening of color
Inactive Publication Date: 2017-01-11
长春市致和生物科技有限公司
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AI Technical Summary
Problems solved by technology
[0002] At present, the method adopted by the hospital for the fixation of small colored specimens within 2 mm of the isolated tissue is to add eosin dye solution to the tissue fixative solution, which can color the small specimens to a certain extent, and can be used to detect tissue before making tissue wax blocks. and identification, while the tissue specimens stained with eosin, after tissue dehydration, transparency, wax immersion, embedding, etc. to form wax blocks, the color of eosin on some small specimens fades or becomes lighter. In the process of making pathological slices from medium-sized tissues, it is not possible to discover and identify small specimens
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 1
[0011] Take out the fresh gastric mucosa under the gastroscope and put it in the tissue fixative for more than 20-30 minutes to achieve the fixed coloring effect. After the routine processing of the pathological samples, the tissue samples in the wax block are obviously colored and easy to find and identify.
Embodiment 2
[0013] Take out the fresh lung tissue under the bronchoscope and put it in the tissue fixative for more than 20-30 minutes to achieve the fixation and coloring effect. After the routine processing of pathological samples, the tissue samples in the wax block are obviously colored and easy to find and identify.
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The invention discloses a formula of tissue fixing liquid for coloring tissue and a usage method thereof. The invention aims at solving problems that small specimens in a process of slicing are not easy to discover and identify due to faded or shallow colors, after the small tissue specimens are prepared into wax blocks with eosin-containing tissue fixing liquid used in hospital at present. The invention discloses the reasonable formula of fixing liquid for coloring tissue and the usage method thereof, and main components comprise magenta, formalin, acetate acid gracial, alcohol, etc. The formula is suitable for pathological slide preparation of small specimens within 2mm. The fixing liquid has the characteristics of short specimen fixing time, good fixing effects, more stable coloring of tissue specimens, and the like, and specimens are easy to discover and identify.
Description
technical field [0001] The invention relates to a pathological tissue fixative, in particular to a formula of tissue-colored tissue fixative and its application method. Background technique [0002] At present, the method adopted by the hospital for the fixation of small colored specimens within 2 mm of the isolated tissue is to add eosin dye solution to the tissue fixative solution, which can color the small specimens to a certain extent, and can be used to detect tissue before making tissue wax blocks. and identification, while the tissue specimens stained with eosin, after tissue dehydration, transparency, wax immersion, embedding, etc. to form wax blocks, the color of eosin on some small specimens fades or becomes lighter. In the process of making pathological slices from medium-sized tissues, it does not play a role in the discovery and identification of small specimens. Contents of the invention [0003] In order to better discover and identify small specimens withi...
Claims
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IPC IPC(8): G01N1/30
Inventor 不公告发明人
Owner 长春市致和生物科技有限公司
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