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Kit for lipolysis by means of light radiation

A technology of lipolysis and light irradiation, which is applied to the parts of surgical instruments, phototherapy, trocars, etc., can solve the problems of insufficient effect, no promotion of lipolysis, side effects, etc.

Inactive Publication Date: 2017-03-01
AMOREPACIFIC CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, although ingredients obtained from natural extracts are used to promote lipolysis, the effect is not necessarily sufficient, and some of them have undesirable side effects
Also, little research has been done to promote lipolysis at the adipocyte level

Method used

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  • Kit for lipolysis by means of light radiation
  • Kit for lipolysis by means of light radiation
  • Kit for lipolysis by means of light radiation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] [Example 1] Light irradiation of the whole stage

[0069] 1-1. Culture of undifferentiated adipocytes

[0070] Undifferentiated mouse 3T3-L1 fibroblasts were obtained from ATCC (CL-173). By using DMEM (Dulbecco's modified Eagle's medium) (PAA, Austria) containing 4.5 g / L glucose supplemented with 10% calf serum (Gibco BRL, NY) in 10% CO 2 Undifferentiated cells (preadipocytes) were cultured in a humid incubator in the USA).

[0071] 1-2. Differentiation into adipocytes

[0072] To differentiate undifferentiated adipocytes obtained in 1-1, the medium was replaced with supplemented with 10% fetal bovine serum (FBS; PAA, Austria), 10 mg / mL insulin (Sigma-Aldrich, St. Louis, USA), 0.5mM 3-isobutyl-1-methylxanthine (IBMX; Sigma-Aldrich, St.Louis, USA) and 1mM dexamethasone (DEX; Sigma-Aldrich, St.Louis, USA) were used for differentiation. Combined media. After 2 days of culture, the medium was replaced with DMEM containing 4.5 g / L glucose supplemented with 10% FBS and 10 ...

Embodiment 2

[0080] [Example 2] Light irradiation after differentiation

[0081] 2-1. Culture of undifferentiated adipocytes

[0082] Undifferentiated cells were obtained in the same manner as in Example 1-1.

[0083] 2-2. Differentiation into adipocytes

[0084] Undifferentiated cells were differentiated into adipocytes in the same manner as in Example 1-2.

[0085] 2-3. Light irradiation

[0086] The differentiated adipocytes obtained in 2-2 were submerged in PBS and incubated with λ in the same nutrient-limited medium as in 1-4. max Irradiate for 6 hours with light of 660 nm, 740 nm, 850 nm, 940 nm, 1050 nm, 1150 nm, 1250 nm or 1350 nm. During light irradiation, the irradiation distance is 5cm and the irradiation intensity is 10J / cm 2 .

[0087] 2-4. Recovery of medium for measurement of triglyceride and glycerol

[0088] According to the same manner as in Example 1-4, from the control group (CTL) and with λ max Culture medium was recovered from test groups treated with light of...

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PUM

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Abstract

This specification provides a light source radiation apparatus for radiating light, a kit for lipolysis having a manual describing a method for dissolving fat by radiating light, and a slimming method for radiating light to skin. According to the specification, the present invention can dissolve neutral fat in a fat cell by radiating light of a wavelength in a range of light harmless to the body, thus can safely and effectively dissolve fat, and can be used for the slimming method by having the advantage of being more economical and convenient than a conventional lipolysis method.

Description

technical field [0001] The present invention relates to a kit for fat decomposition and a slimming method. Background technique [0002] Obesity results from the accumulation of excess energy in fat cells as triglycerides. Since obesity can cause various diseases including arteriosclerosis and is aesthetically unfavorable, prevention and improvement of obesity are strongly desired. [0003] Recently, due to reasons such as overeating, lack of exercise, stress, etc., the number of obese people is increasing every year all over the world, especially in developed countries. They often forego losing weight or exercising because of the willpower and long hours required. [0004] Therefore, efforts are being made to develop methods for lipolysis or agents for promoting lipolysis in order to prevent and improve obesity. For example, although ingredients obtained from natural extracts are used to promote lipolysis, the effects are not necessarily sufficient, and some of them have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61N5/06A61B18/00
CPCA61N5/0616A61B18/203A61B2018/00464A61B2018/2005A61N2005/0612A61N2005/0643A61N2005/0659A61N2005/0662A61B18/20A61N5/0613A61B18/22A61B2018/00273A61N5/0601
Inventor 崔敏植金亨俊郑智溶李泰龙申东旭
Owner AMOREPACIFIC CORP
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