Application of substances for detecting sparc protein in serum in preparation of kits for screening hepatocellular carcinoma

A protein and serum technology, applied in the biological field, can solve problems such as opposite expression levels and complexity

Active Publication Date: 2018-05-08
BEIJING PROTEOME RES CENT +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the relationship between the expression levels of each protein in peripheral blood and tissues is very complicated, and its expression level in peripheral blood cannot be simply deduced from the expression level in tissues, and sometimes the expression levels of the two are completely opposite

Method used

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  • Application of substances for detecting sparc protein in serum in preparation of kits for screening hepatocellular carcinoma
  • Application of substances for detecting sparc protein in serum in preparation of kits for screening hepatocellular carcinoma
  • Application of substances for detecting sparc protein in serum in preparation of kits for screening hepatocellular carcinoma

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Experimental program
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Effect test

Embodiment 1

[0041] Embodiment 1, the relationship between SPARC protein in serum and HCC patient

[0042] 1. Experimental materials

[0043] Serum samples were obtained from:

[0044] Normal group: 30 cases of normal people (N);

[0045]LC patient group: 35 patients with liver cirrhosis (not transformed into hepatocellular carcinoma, LC) with HBV chronic infection background;

[0046] HCC patient group: 50 patients with hepatocellular carcinoma (HCC) with HBV chronic infection background (23 of them were APF negative).

[0047] Among them, the serum samples of normal people were from healthy persons; the serum samples of patients with liver cirrhosis and patients with hepatocellular carcinoma were clinically confirmed patients.

[0048] 2. Relationship between SPARC protein in serum and HCC patients

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Abstract

The invention discloses new application of a substance for detecting the concentration of an SPARC (Satraplatin and Prednisone Against Refractory Cancer) protein. The new application provided by the invention is application of a substance for detecting SPARC protein concentration to preparation of a product for screening or helping to screen the hepatocellular carcinoma. An experiment proves that the SPARC protein can be used as a tumor marker of the hepatocellular carcinoma; screening is carried out under the condition of taking normal persons as screening targets, the sensitivity of judging HCC patients is 82%, the specificity is 93% and the AUC (Area Under roc Curve) is 0.89; screening is carried out by taking LC (Liver Cirrhosis) patients as screening targets, the sensitivity of judging the HCC patients is 62%, the specificity is 86% and the AUC is 0.73; the SPARC protein can be used for diagnosing AFP (Alpha-fetoprotein) negative hepatocellular carcinoma; and the SPARC protein can also be combined with AFP to be used for screening or helping to diagnose the HCC from the normal persons or the LC patients.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of a substance for detecting SPARC protein in serum in the preparation of a kit for screening hepatocellular carcinoma. Background technique [0002] Hepatocellular carcinoma (hepatocellular carcinoma, HCC) is one of the common malignant tumors with high morbidity and mortality. About 650,000 people die from HCC every year, and the incidence rate tends to increase. The disease occurs mainly in Southeast Asia, and more than 50% of it occurs in China. The occurrence of hepatocellular carcinoma is a multi-factor and multi-stage process. Chronic infection of hepatitis B virus (HBV) is an important high-risk factor. Patients with liver cirrhosis (LC) with hepatitis B background It is a high-risk group that may develop liver cancer. At present, the 5-year survival rate of hepatocellular carcinoma is poor. Early screening of hepatocellular carcinoma, especially screening o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574
CPCG01N33/56966G01N33/57438G01N33/57476G01N33/57488
Inventor 贺福初孙薇张剑孙龙钦邢宝才牟劲松赵晓航
Owner BEIJING PROTEOME RES CENT
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