Method for identifying wine country on basis of metabonomics research means

A wine and origin technology, applied in the field of wine origin identification, can solve the problems of unexplored column changes, etc., and achieve the effect of accurate and simple detection method, small external environment interference, and simple pretreatment method

Inactive Publication Date: 2017-05-31
JIANGNAN UNIV
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Problems solved by technology

The detection of mineral elements is based on the different substances absorbed from the soil environment by the grapes in different production areas during t...

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  • Method for identifying wine country on basis of metabonomics research means
  • Method for identifying wine country on basis of metabonomics research means
  • Method for identifying wine country on basis of metabonomics research means

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Embodiment 1

[0041] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0042] A) Choose the wines of France, Chile, Australia and Spain as the experimental research objects.

[0043] B) Adsorbing the sample by solid-phase microextraction;

[0044] (1) Take 5 mL of the sample to be tested and place it in a 20 mL headspace vial, and add 10 μL of 2-octanol with a concentration of 100 μg / mL as an internal standard and 1.2 g of NaCl as an activator, and add a magnetic stirrer, and cover tightly Shake the cap well.

[0045] (2) Add an appropriate amount of water to the IKA magnetic stirrer and set the temperature at 45°C to keep it constant, immerse the sample bottle in warm water at 45°C and keep the stirring speed of the magnetic stirrer at 300rmp, and preheat for 5 minutes. After preheating, immediately insert the extraction head into the sample bottle through the cap of the sample bottle, and suspend it in the hea...

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Abstract

The invention discloses a method for identifying a wine country on the basis of a metabonomics research means, and belongs to the technical field of wine country identification. The method comprises the main steps of: (1) extracting a volatile component of a wine sample by utilizing a solid-phase micro extraction technology; (2) analyzing the volatile component of wine by utilizing a gas chromatography-mass spectrometry combined technology to obtain a reconstructed total ion chromatogram; (3) carrying out retrieval and analysis by NIST2014, WILEY8.0 spectrum library to obtain compound information corresponding to each peak, and representing relative content of each substance with a ratio of an area of each compound peak to an area of an internal labeled 2-octanol peak; (4) establishing PCA and OPLS-DA models by utilizing SIMCA13.0, and finding out different metabolism. The wine country tracing method disclosed by the invention is simple to preprocess, simple and convenient to operate, high in identification rate and beneficial to health development of the wine market.

Description

technical field [0001] The invention relates to a method for identifying wine production areas based on metabolomics research means, and belongs to the technical field of wine production area identification. Background technique [0002] Wine is an alcoholic drink with high nutritional value, and drinking it in moderation is good for your health. In recent years, with the improvement of people's living standards in our country, the import volume of wine has also increased. Well-known wine producing areas are mainly concentrated in Europe, and the southern European producing areas are the most densely distributed, so they have also become the main research object of wine producing areas identification. The climate, soil, picking conditions, and winemaking techniques of different producing areas have resulted in the unique flavor of wine and the diversification of wine varieties, making the identification of its producing area an important commodity value. Therefore, some un...

Claims

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Application Information

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IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/884
Inventor 顾小红王怡淼王利平胡博张慧赵建新
Owner JIANGNAN UNIV
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