Primers, kits, methods and applications for detecting miRNA expression associated with colorectal cancer vincristine drug resistance

A technology for vincristine and colorectal cancer, applied in biochemical equipment and methods, DNA/RNA fragments, drug combinations, etc., can solve problems such as VCR drug resistance, reduce side effects, improve curative effect, and improve chemotherapy effects Effect

Active Publication Date: 2020-01-24
河南普诺易生物制品研究院有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the course of use, tumor cells will gradually develop drug resistance to VCR

Method used

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  • Primers, kits, methods and applications for detecting miRNA expression associated with colorectal cancer vincristine drug resistance
  • Primers, kits, methods and applications for detecting miRNA expression associated with colorectal cancer vincristine drug resistance
  • Primers, kits, methods and applications for detecting miRNA expression associated with colorectal cancer vincristine drug resistance

Examples

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Embodiment 1

[0050] The primers used to detect the miRNA expression associated with colorectal cancer vincristine drug resistance are designed and synthesized according to the miRNA sequence (as shown in SEQ ID NO: 1), including reverse transcription primers and fluorescent quantitative PCR primers. The reverse transcription primers are as follows: As shown in SEQ ID NO: 2, 3 or 4, the forward primer of the fluorescent quantitative PCR primer is shown in SEQ ID NO: 5 or 6, and the reverse primer is shown in SEQ ID NO: 7.

Embodiment 2

[0052] The kit for detecting miRNA expression related to colorectal cancer vincristine drug resistance, comprising the primers in Example 1, miRNA reverse transcription reagents and fluorescent quantitative polymerase chain reaction reagents.

Embodiment 3

[0054] The kit for detecting the expression of miRNA associated with colorectal cancer vincristine drug resistance, including primers in Example 1, reverse transcription primers and fluorescent quantitative PCR primers for internal reference U6snRNA, and DNA digestion reagents (DNase I (RNase-free ), EDPC, etc.), miRNA reverse transcription reagents (reverse transcriptase, RT buffer, dNTPmix, etc.) and fluorescent quantitative polymerase chain reaction reagents (dNTPmix, SYBR Green PCR Master Mix, etc.).

[0055] The reverse transcription primer of internal reference U6snRNA is shown in SEQ ID NO: 8, and the forward and reverse primers of fluorescent quantitative PCR are shown in SEQ ID NO: 9 and 10, respectively.

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Abstract

The invention discloses a primer, a kit, a method and an application for detecting miRNA expression related to colorectal cancer vincristine drug resistance, and belongs to the technical field of tumor biotherapy. The present invention confirms through experiments for the first time that the miRNA sequence shown in SEQ ID NO: 1 is related to the drug resistance of vincristine, and miRNA is highly expressed in vincristine drug-resistant cells and lowly expressed in sensitive cells, so it can be used as a tumor cell vincristine markers of drug resistance. The primers, kits and detection methods designed based on the marker can be used to clinically judge the drug resistance of tumor cells to vincristine, further guide clinical medication and achieve precise treatment, improve curative effect and reduce side effects. The invention provides a new target for designing the vincristine drug resistance of tumor cells, and can be used for directly developing drugs for antagonizing the vincristine drug resistance of tumor cells.

Description

technical field [0001] The present invention relates to a primer for detecting the expression of miRNA associated with colorectal cancer vincristine drug resistance, as well as a kit and a detection method comprising the primer, and the use of the miRNA in the preparation of drugs for antagonizing tumor vincristine resistance The application in the invention belongs to the technical field of tumor biotherapy. Background technique [0002] Malignant tumors are common and frequently-occurring diseases that seriously threaten human health, and colorectal cancer (CRC) is one of the common malignant tumors of the digestive system, ranking first in cancer incidence and second in mortality in developed countries. It ranks the 4th in my country's cancer mortality rate, and its age of onset tends to be aging. Recent epidemiological data show that the number of new cases of CRC worldwide accounts for 9.4% of all new cases of malignant tumors. With the development of the economy, the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11A61K31/7105A61P35/00
CPCC12Q1/6886C12Q2600/106C12Q2600/178C12Q2600/158
Inventor 董卫华王天云张俊河高建辉姚朝阳倪天军
Owner 河南普诺易生物制品研究院有限公司
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