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A kind of breeding method of two-line male sterile line of loose panicle type aromatic rice

A technology of sterile line and fragrant rice, which is applied in the direction of botany equipment and methods, plant gene improvement, application, etc., to achieve high efficiency, good benefit, and stable sterility

Active Publication Date: 2018-10-23
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the panicle types of these aromatic rice strains bred in China are dense, and there is no report on the two-line male sterile line of the fragrant rice with loose panicle type.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] a) During the fertile period of the two-line CMS line, select the two-line CMS line SE21s (P1), a loose-eared rice line spr8 (P2) As a hybrid parent, cross P1 / P2 to get hybrid F 1 Seed to F 1 -12 means;

[0022] b) planting F 1 -12 plants with 40 plants. After eliminating false hybrids at the heading stage, the fragrant rice line Luxiang 618 (P3) was selected as a three-cross parent to make a three-cross combination P1 / P2 / / P3, and a three-cross F 1 Substituting seeds, with three-cross F 1 -123 means;

[0023] c) During the sterile period of the two-line sterile line, plant three-cross F 1-123 plants with 3820 plants. During the heading and flowering stage, pollen microscopic examination was carried out on the separated sterile single plants with loose ears, and the single plants with a sterility degree of 100% were selected; and identification and screening were carried out according to the target traits that the two-line sterile lines should have. At the same ...

Embodiment 2

[0030] a) During the fertile period of the two-line CMS line, select the two-line CMS line Guangzhan 63s (P1), a loose panicle rice line spr8 (P2) As a hybrid parent, cross P1 / P2 to get hybrid F 1 Seed to F 1 -12 means;

[0031] b) planting F 1 -12 plants 40-45 plants. After eliminating false hybrids at the heading stage, the fragrant rice line Luxiang 618 (P3) was selected as a three-cross parent to make a three-cross combination P1 / P2 / / P3, and a three-cross F 1 Substituting seeds, with three-cross F 1 -123 means;

[0032] c) During the sterile period of the two-line sterile line, plant three-cross F 1 -123 plants with 3760 plants. During the heading and flowering stage, pollen microscopic examination was carried out on the separated sterile single plants with loose ears, and the single plants with a sterility degree of 100% were selected; and identification and screening were carried out according to the target traits that the two-line sterile lines should have. At ...

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PUM

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Abstract

The invention discloses a breeding method for a loose spike type rice two-line sterile line and belongs to the technical field of breeding of a special rice sterile line. The method comprises the following steps: taking rice two-line sterile line (one line for dual purpose), loose spike type rice strain and fragrant rice strain as cross parents, adopting a triple-crossing hybrid combining method for planting under different light and temperature conditions in the sterile period and fertile period of the two-line sterile line, identifying multi-generation spikes, fragrance, sterility and fertility and screening the comprehensive characters, thereby breeding the excellent loose spike type rice two-line sterile line with loose spike form, thick aromatic flavor of rice, sterility stability, high combining ability, excellent breeding, seed production and high hybrid yield. The hybrid seed prepared from the sterile line is high in outcrossing rate, high in seed multiplication capacity, excellent rice appearance and high eating quality; the spike diseases, such as, false smut, can be obviously reduced; the dosage of the pesticide can be reduced and the peasant income can be increased.

Description

technical field [0001] The invention belongs to the technical field of special rice sterile line breeding, and in particular relates to a method for selecting a two-line sterile line of loose panicle type aromatic rice. Background technique [0002] The combination of ideal plant type and utilization of heterosis is one of the important ways to achieve a breakthrough in rice yield. The issue of panicle type has always been the focus of rice ideal plant type breeding and cultivation research. Panic type is closely related to rice yield level and population structure. relation. According to the branching pattern of the panicle, the angle of the primary branch, the density of spikelets and the spatial posture, the panicle type can be divided into compact panicle, spreading panicle and intermediate type. (Intermediate panicle). Up to now, a total of 7 panicle trait genes have been reported, among which, spr1 and spr8 It is controlled by a recessive nuclear gene, and the oth...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 梁康迳梁义元陈芳育郭玉春郑秀娟
Owner FUJIAN AGRI & FORESTRY UNIV