Mouse model with lung metastasis of gastric cancer and establishment method thereof

Abstract

The invention relates to a mouse model with lung metastasis of gastric cancer and an establishment method thereof, and belongs to the technical field of experimental animals. According to the mouse model and the establishment method thereof, BGC-823 and MKN-45 gastric cancer cells are cultured in vitro, and resuspended to a proper concentration with sterile PBS when the cell viability is strong. After the cells are cultured inside the body of immunodeficient NUDE and SCID mice inoculated in immunodeficience through the caudal vein, the mouse model with lung metastasis of gastric cancer is successfully obtained by the identifications including the statistics of surface lung nodules, HE staining and IHC staining. According to the mouse model establishment, needed experimental conditions and operation are simple and convenient, lung metastasis lesions after the experiment are obvious, metastasis efficiency is high, and mice with metastatic lesions have moderate survival cycles. In the evaluation and statistics of the metastasis results, the macroscopic counting of the lung nodules is improved into the microscopic counting of the IHC staining positive cells, thereby obtaining more objective and accurate results. The animal model of tumor metastasis may be widely used in scientific research and teaching.

Description

technical field [0001] The invention relates to a mouse model of lung metastasis of gastric cancer and a method for establishing the same, belonging to the technical field of experimental animals. Background technique [0002] Gastric cancer (GC) is one of the fifth most common malignant tumors (lung cancer, bowel cancer, breast cancer, liver cancer) in the world. In developed countries in the world, gastric cancer ranks fifth in the number of new cancers, after lung cancer, breast cancer, colon cancer, and liver cancer; in developing countries, it ranks second, second only to lung cancer. According to foreign literature statistics, gastric cancer accounts for 50% of digestive system tumors, and domestic reports account for 62%. In 2012, there were 952,000 new cases (7% of all cancers) and 723,000 deaths (9% of all cancers). In my country, there are 300,000 newly diagnosed gastric cancers every year, and as many as 160,000 people die from gastric cancer, accounting for 23%...

AI Technical Summary

Problems solved by technology

Although the transplanted subcutaneous tumor can invade the muscle layer, it is difficult to penetrate the muscle septum, and it is difficult to accurately count the cases of metastasis to the muscle surface

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