Method for determining content of toddalolactone in Liangmianzhen Chinese medicinal toothpaste by using RP-HPLC

A technology of RP-HPLC and L. japonica, which is applied in the field of chemical detection, can solve the problems of content and authenticity effects, imperfect quality control indicators of L. japonicus, etc., and achieves good precision and repeatability, little damage, and separation effect. Good results

Inactive Publication Date: 2017-08-22
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, the quality control indicators of LMZ have not been perfected, and the quality of LMZ herbs used in the preparation of LMZ toothpaste may vary. However, the formulation process of LMZ toothpaste is strictly kept secret, and there is no relevant report on its quality standard. Therefore, the selection of the raw material drug LMZ will also affect the content and authenticity of the effective components of LMZ toothpaste.

Method used

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  • Method for determining content of toddalolactone in Liangmianzhen Chinese medicinal toothpaste by using RP-HPLC
  • Method for determining content of toddalolactone in Liangmianzhen Chinese medicinal toothpaste by using RP-HPLC
  • Method for determining content of toddalolactone in Liangmianzhen Chinese medicinal toothpaste by using RP-HPLC

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Embodiment 1

[0042] 1 Instruments and reagents

[0043] 1.1 Instrument

[0044] Agilent bio-inert quaternary liquid chromatograph (instrument model: Agilent 1260; manufacturer: Agilenttechnology);

[0045] Diode Array Detector (DAD);

[0046] Electronic analytical balance (instrument model: CPA225D; manufacturer: Sartorius Scientific Instruments (Beijing) Co., Ltd.);

[0047] Ultrasonic cleaner (instrument model: Elmasonic P180H; manufacturer: Elma Schmidbauer GmbH, Germany);

[0048] Ultrapure water meter (instrument model: ELGAPURELAB ClassicUVF; manufacturer: Veolia Group);

[0049] Heating magnetic stirrer (instrument model: C-MAG HS 7; manufacturer: IKA, Germany);

[0050] High-performance general-purpose desktop centrifuge (instrument model: AllegraX-15R; manufacturer: Beckman Company of the United States);

[0051] Desktop refrigerated centrifuge (instrument model: 3K15; manufacturer: Sigma, Germany).

[0052] 1.2 Reagent

[0053] Maoliangmianzhen reference substance (provide...

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Abstract

The invention discloses a method for determining the content of toddalolactone in Liangmianzhen Chinese medicinal toothpaste by using RP-HPLC, belonging to the field of chemical detection. The method has the advantages of simple operation, rapidness, accuracy, low interference, good precision and repeatability, etc. According to the method, methanol is employed for extracting Zanthoxylum nitidum var. tomentosum in the Liangmianzhen Chinese medicinal toothpaste, and methanol extract is collected; and the content of toddalolactone in the methanol extract is determined by using RP-HPLC. The method can provide experimental bases for determination of the content of toddalolactone in the Liangmianzhen Chinese medicinal toothpaste.

Description

technical field [0001] The invention belongs to the field of chemical detection, in particular to a RP-HPLC method for measuring the content of lianmianzhen in liangmianzhen traditional Chinese medicine toothpaste. Background technique [0002] Liangmianzhen is the dried root of Zanthoxylum nitidum (Roxb.) DC., a Rutaceae plant. It can promote blood circulation and remove blood stasis, promote qi and relieve pain, dispel wind and collaterals, detoxify and reduce swelling. Do not overdose [1] , the variant of Maoliangmianzhen [2] , LMZ medicinal materials are different from Mao LMZ in terms of chemical composition and content. Maoliangzhensu, also known as Feilongzhang blood lactone, is a unique component of Maoliangzhen and Feilongzhang Xuegen; Lianmianzhen chloride is one of the main active ingredients of Liangmianzhen; it exists in medicinal materials Liangmianzhen, Maoliangmianzhen and Feilongzhang in the blood, but the content is different among the three [3-5] . "Ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 林昊冯泽川张云平陈学松黄馨慧
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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