Gas chromatography-mass spectrometry screening method for unknown toxicants in blood

Abstract

The invention discloses a method for gas chromatograph-mass spectrometry screening of unknown toxins in blood. The method includes: taking to-be-detected blood, pretreating, performing GC-MS (gas chromatograph-mass spectrometry) analysis, and judging whether a sample of the to-be-detected blood contains toxin components or not according to analysis results, wherein a pretreatment method refers to a liquid-liquid extraction method or a solid-liquid extraction method. The liquid-liquid extraction method includes: (1) when target detect substances are toxins, adding heptachlor epoxide operating fluid, and performing ethyl acetate: benzene extraction; (2) when target detect substances are drugs, adding SKF525A and aprobarbital internal standard operating fluid, and performing ethyl acetate: benzene extraction. A method for detecting different types of toxins is established, quickness and accuracy in detection are achieved, the pretreatment method is optimized, detection limits of common toxins are given, high-sensitivity effective detection of various targets is guaranteed, a scientific data support is provided for negative test results, and a powerful scientific basis is provided for related clinical diagnosis, emergency treatment, criminal investigation and criminal proceedings.

Description

technical field [0001] The invention relates to a gas chromatography-mass spectrometry screening method for unknown poisons in blood, and belongs to the technical field of chemical substance detection. Background technique [0002] Common poison screening occupies a considerable proportion in forensic science physical and chemical testing. Through the investigation of drug-related cases in Shandong Province in the past 10 years, the applicant found the following problems in the physical evidence examination of such cases: [0003] (1) The types of poisons involved in the case have increased significantly. In the investigation of some cases where the poisoning is not clear, the poisons that need to be screened are not limited to a certain type or a certain type, that is, the target is not strong. In this case, if the old method is still used to screen for only a few of the most common toxicants, it is very likely to cause missed detection. [0004] (2) At present, the GC me...

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Problems solved by technology

[0003] (1) The types of poisons involved in the case have increased significantly. In the investigation of some cases where the poisoning is not clear, the poisons that need to be screened are not limited to certain types or types, that is, the target is not strong

In this case, if the old method is still used to screen for only a few of the most common toxicants, it is very likely to cause missed detection

[0004] (2) At present, the GC method is an instrumental analysis method generally mastered and adopted by all cities in the province. The corresponding sample pretreatment method of this method is not standardized and has not been optimized.

[0005] (3) The use of GC-MS to systematically examine the detection limits of the toxicants involved in the case is still blank, and the evaluation of negative results lacks scientific data support

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