Liquid chromatography-mass spectrometry screening method of unknown toxic substances in blood

Abstract

The invention discloses a liquid chromatography-mass spectrometry screening method of unknown toxic substances in blood. The liquid chromatography-mass spectrometry screening method comprises the following steps: taking a blood sample to be detected, adding acetonitrile precipitated protein, oscillating, centrifuging, taking a supernatant, filtering by using a 0.22-micron microporous organic filter membrane, then performing LC-MS / MS analysis, and judging whether the blood sample to be detected contains toxic substance components or not according to an analysis result; the used instrument is an ultraLC 100-XL-4000Q TRAP liquid chromatograph / mass spectrometer or an Agilent 1100 liquid chromatography-AB 3200 QTRAP triple serial quadrupole mass spectrometer from an AB company. By the liquid chromatography-mass spectrometry screening method, detection methods of different types of toxic substances are established, detection is fast and accurate, the detection limits of common toxic substances are given, high-sensitivity and effective detection of all target objects are guaranteed, a scientific data support is provided for a negative detection result, and thus a strong scientific basis is provided for relevant clinical diagnosis and rescue as well as criminal investigation and litigation.

Description

technical field [0001] The invention relates to a liquid chromatography-mass spectrometry screening method for unknown poisons in blood, and belongs to the technical field of chemical substance detection. Background technique [0002] Common poison screening occupies a considerable proportion in forensic science physical and chemical testing. Through the investigation of drug-related cases in Shandong Province in the past 10 years, the applicant found the following problems in the physical evidence examination of such cases: [0003] (1) The types of poisons involved in the case have increased significantly. In the investigation of some cases where the poisoning is not clear, the poisons that need to be screened are not limited to a certain type or a certain type, that is, the target is not strong. In this case, if the old method is still used to screen for only a few of the most common toxicants, it is very likely to cause missed detection. [0004] (2) At present, the GC...

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Problems solved by technology

[0003] (1) The types of poisons involved in the case have increased significantly. In the investigation of some cases where the poisoning is not clear, the poisons that need to be screened are not limited to certain types or types, that is, the target is not strong

In this case, if the old method is still used to screen for only a few of the most common toxicants, it is very likely to cause missed detection

[0004] (2) At present, the GC method is an instrumental analysis method generally mastered and adopted by all cities in the province. The corresponding sample pretreatment method of this method is not standardized and has not been optimized.

[0005] (3) Due to the limitation of the performance of the instrument itself, the GC method cannot be used for highly sensitive and effective detection of certain poisons

Although various cities have successively introduced liquid mass spectrometry equipment, systematic method development has not been carried out, and liquid mass spectrometry technology cannot be fully utilized to improve the sensitivity of toxicant detection

[0006] (4) Although the application of liquid chromatography-mass spectrometry in toxicant screening has been reported, the pretreatment steps are too complicated, and all analytes are analyzed under uniform liquid phase conditions, and the effect is not ideal

At the same time, due to differences in instrument brands and models, relevant conditions and data cannot be directly used as the basis for forensic toxicology screening in this province

[0007] (5) The systematic examination of the detection limits of the toxicants involved in the case by liquid chromatography-mass spectrometry is still blank, and the evaluation of negative results lacks scientific data support

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