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Rapid separation and analysis methods of iridoids (glycosides) compounds in Xinba and their application in hypoglycemic drugs

A technology for iridoid glycosides and iridoids is applied in the application field of preparing hypoglycemic drugs, can solve the problems of weak ultraviolet absorption, poor pertinence, difficult quantitative analysis and the like, and achieves high separation efficiency, The analysis results are reliable, and the effect is conducive to quantitative comparison.

Active Publication Date: 2020-09-22
XIAN UNIV OF SCI & TECH
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Problems solved by technology

This extraction method is suitable for the separation of small molecular compounds in natural products, but the pertinence is poor, the extraction method is complicated, and the overall efficiency is low
Simultaneously, because of the special structure of iridoid (glycoside) compound, the difficulty of quantitative analysis is very large: if use external standard method, need the standard sample of every kind of iridoid (glycoside) compound, difficulty is extremely high; With the internal standard method, because of its weak ultraviolet absorption, the error is large

Method used

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  • Rapid separation and analysis methods of iridoids (glycosides) compounds in Xinba and their application in hypoglycemic drugs
  • Rapid separation and analysis methods of iridoids (glycosides) compounds in Xinba and their application in hypoglycemic drugs
  • Rapid separation and analysis methods of iridoids (glycosides) compounds in Xinba and their application in hypoglycemic drugs

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Embodiment Construction

[0030] The present invention will be further described below in conjunction with drawings and embodiments.

[0031] (1) Ultrasonic extraction of core bar

[0032] Take 100 grams of core bark (water content lower than 0.5%), pulverize, pass through a 20-mesh sieve to obtain coarse powder, add coarse powder to 400mL mixed solvent (ethyl acetate 200mL+acetone 100mL+methanol 100mL, 1:0.5:0.5), in Ultrasonic extraction in a water bath at 40°C for 60min (ultrasonic power 150W), filtered, the extract (i.e. filtrate) was concentrated (60°C, -0.1MPa) and then freeze-dried (-80°C, -0.09MPa) to obtain 8.6g of the extract, The total extraction rate was 8.6%.

[0033] (2) Qualitative and quantitative analysis of iridoids (glycosides) compounds in Xinba

[0034] Take 25mg of the above extract and dissolve it in 0.5mL CDCl 3 , then add 1M phloroglucinol standard solution (solvent is CDCl 3 ) 5 μL to get the sample, Bruker AM 400MHz to measure the hydrogen resonance signal of the sample, ...

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Abstract

The invention discloses a rapid separation and analysis method of iridoid (glycoside) compounds in cymbaria and an application of the iridoid (glycoside) compounds in cymbaria in sugar reducing drugs. A mixed solvent is adopted to directly extract cymbaria, the content of the iridoid (glycoside) compounds in a cymbaria extract are qualitatively and quantitatively analyzed with an NMR technology, and the technical problems of low extraction and separation efficiency, larger effective component content determination errors and tedious steps in the prior art are solved. The separated extract containing the iridoid (glycoside) compounds has significant in-vivo sugar reducing effect, can remarkably improve sugar tolerance of diabetes mice in STZ and alloxan induced diabetes mouse models and can be applied to preparation of the sugar reducing drugs.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to the separation and analysis of iridoid (glycoside) compounds (i.e. iridoids and iridoid glycoside compounds) of the active component of Sinba and its use in the preparation of hypoglycemic drugs Applications. Background technique [0002] Cymbaria dahurica L. and Cymbaria mongolica Maxim. are the dried whole plants of Scrophulariaceae (Scrophulariaceae). The Mongolian medicine volume of "Chinese Materia Medica" records that Xinba has a history of more than 700 years of application. It is used as the main drug or formula in classic Mongolian medicine prescriptions such as Siwei Xinba San and Bawei Xinba San. Li Minhui et al. found that the n-butanol extract of Xinba can dose-dependently reduce the blood sugar level of alloxan-induced diabetic mice, while the water extract and low-dose n-butanol extract of Xinba have little effect on blood sugar. The research of Wu Xiaol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/80A61P3/10G01N24/08
CPCA61K36/80A61K2236/333A61K2236/51G01N24/082
Inventor 陈福欣龚频加晓丹侯彬彬陈苏英严贝贝杨倩倩
Owner XIAN UNIV OF SCI & TECH
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