Experimental mouse model with fecal microbiota transplantation and construction method

A fecal-bacteria transplantation and mouse model technology, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, and active ingredients of artificially synthesized polymeric materials, etc., can solve the problems of cumbersome construction, difficulty in real response, poor model effect, etc. , to reduce costs

Pending Publication Date: 2017-11-17
THE FIFTH AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the germ-free mouse model of the fecal bacteria transplantation experiment is not only

Method used

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  • Experimental mouse model with fecal microbiota transplantation and construction method
  • Experimental mouse model with fecal microbiota transplantation and construction method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The construction of the experimental mouse model of embodiment 1 fecal bacteria transplantation

[0025] 1) The mice were intragastrically injected with vancomycin saline solution twice a day for 3 consecutive days; the vancomycin saline solution for each intragastric injection was 0.25ml, and the amount of vancomycin in the vancomycin saline solution was 0.25ml. The element concentration is 20mg / ml;

[0026] 2) After intragastrically injecting vancomycin saline solution into the mice, they drink the compound polyethylene glycol saline solution as the water source, and the mice drink it for 3 consecutive days;

[0027] 3) After the mice drank the compound polyethylene glycol saline solution, the intestinal flora of the stroke patients were used to perform fecal bacteria transplantation on the mice, and an experimental mouse model of fecal bacteria transplantation was constructed.

Embodiment 2

[0029] Taking the experimental mouse model of fecal bacteria transplantation in Example 1 as the object (assuming a group of blank controls), the middle section of the colorectum tissue is carried out pathological detection, and pathological detection comprises the following steps:

[0030] 1) Specimen collection: Mice were fasted for 12 hours before specimen collection, and had free access to water. The mice were sacrificed by disabling the cervical vertebrae, soaked in 75% ethanol for 3 minutes, and the mice were taken out and placed on sterile gauze in a supine position. Make an incision in the middle of the mouse abdomen, cut the skin, expose the abdominal wall, lift the peritoneum and cut it open. Expose the abdominal cavity tissue, carefully divide the entire intestinal tube from the ileocecal to the abdominal door, wash the intestinal cavity with ice saline, wash away the intestinal content, and retain the colonic tissue and fix it with 10% formalin;

[0031] 2) Fabric...

Embodiment 3

[0037] Taking the experimental mouse model of fecal bacteria transplantation in Example 1 as the object (set up a group of blank controls), the intestinal flora in the cecum stool was detected by 16S rRNA gene sequencing. The experimental results are as follows figure 2 As shown, it was found that the intestinal flora of experimental mice transplanted with fecal bacteria changed significantly. It can be seen that the experimental mouse model of fecal bacteria transplantation of the present invention is successfully constructed.

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Abstract

The invention discloses an experimental mouse model with fecal microbiota transplantation and a construction method. The construction method comprises the following steps that intragastric injection with an antibiotic solution is conducted on a mouse, the number of times of intragastric injection is at least one time a day, and the number of days of intragastric injection is at least one day; after intragastric injection with the antibiotic solution is conducted on the mouse, a laxative solution is taken as a water resource for drinking, and the number of days of drinking the laxatives solution is at least one day; and after the mouse drinks the laxative solution, fecal microbiota transplantation is conducted on the mouse, specifically, the experimental mouse model with fecal microbiota transplantation is constructed. According to the experimental mouse model with fecal microbiota transplantation and the construction method, the construction method is simple, and situation can be truly and reliably reflected.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an experimental mouse model of fecal bacteria transplantation and a construction method thereof. Background technique [0002] Recently, modern medical schools have increasingly recognized the important role of intestinal bacteria in the human body, and maintain or rebuild the normal intestinal flora of the human body as an important treatment for many diseases. As a treatment for intestinal and extra-intestinal diseases, Fecal Microbiota Transplantation (FMT) is to transplant functional flora from human healthy feces into the gastrointestinal tract of patients to rebuild new intestinal flora. Fecal microbiota transplantation has been paid more and more attention, but it has some shortcomings, so it is necessary to construct an experimental mouse model for in-depth research and analysis. [0003] At present, the germ-free mouse model of the fecal bacteria transplantation...

Claims

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Application Information

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IPC IPC(8): A61K35/741A61K38/14A61K31/77
CPCA61K31/77A61K35/741A61K38/14
Inventor 刘志华刘亭杨彬珧
Owner THE FIFTH AFFILIATED HOSPITAL OF GUANGZHOU MEDICAL UNIV
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